| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22377SS | |||
| Azide and BSA Free | NBP2-80948 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37388-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2220-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2220-SP | |||
![Western Blot: Siglec-3/CD33 Antibody (44M12D3) [NBP2-22377] - Analysis of CD33 in A) a partial recombinant protein and B) HCT-116 lysate using CD33 antibody at 2 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-44M12D3-Western-Blot-NBP2-22377-img0005.jpg "Western Blot: Siglec-3/CD33 Antibody (44M12D3) [NBP2-22377] - Analysis of CD33 in A) a partial recombinant protein and B) HCT-116 lysate using CD33 antibody at 2 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody (44M12D3) [NBP2-22377] - Human lung tissue stained with CD33 antibody (3 ug/ml), peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-44M12D3-Immunohistochemistry-Paraffin-NBP2-22377-img0006.jpg)
![Western Blot: Siglec-3/CD33 Antibody [NBP2-29619] - Analysis of CD33 in the partial recombinant protein.](http://images.novusbio.com/fullsize/CD33-Antibody-Western-Blot-NBP2-29619-img0001.jpg "Western Blot: Siglec-3/CD33 Antibody [NBP2-29619] - Analysis of CD33 in the partial recombinant protein.")
![Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-29619] - Analysis of CD33 protein in a section of human small intestinal malignant stromal tumor using 5 ug/mL concentration of CD33 antibody. The cancer cells developed the expected membrane-cytoplasmic staining pattern.](http://images.novusbio.com/fullsize/CD33-Antibody-Immunohistochemistry-Paraffin-NBP2-29619-img0002.jpg)
![Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Western-Blot-NBP2-37388-img0003.jpg "Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of paraffin-embedded endometrium tissues using CD33 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Immunohistochemistry-NBP2-37388-img0007.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of HepG2 cells using CD33 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Flow-Cytometry-NBP2-37388-img0004.jpg)
![Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against human CD33 recombinant protein. (Expected MW is 49.2 kDa)](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Western-Blot-NBP2-37388-img0002.jpg)
![Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of paraffin-embedded esophageal cancer tissues using CD33 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-Immunohistochemistry-NBP2-37388-img0005.jpg)
![ELISA: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-2C6B7-ELISA-NBP2-37388-img0001.jpg)
![Western Blot: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356] - Antibody: Rabbit anti-CD33 recombinant monoclonal [BLR061G] (NBP2-77356). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-BLR061G-Western-Blot-NBP2-77356-img0002.jpg "Western Blot: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356] - Antibody: Rabbit anti-CD33 recombinant monoclonal [BLR061G] (NBP2-77356). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.")
![Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356] - Section of Acute Myeloid Leukemia (AML). Antibody: Rabbit anti-CD33 recombinant monoclonal antibody [BLR061G] (A700-061 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-BLR061G-Immunohistochemistry-Paraffin-NBP2-77356-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356] - Detection of human CD33 by WB of immunoprecipitates from HEL 92.1.7 lysate. Antibodies: Rabbit anti-CD33 recombinant monoclonal [BLR061G] (NBP2-77356) and rabbit anti-CD33 recombinant monoclonal [BL-302-3E10].](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-BLR061G-Immunoprecipitation-NBP2-77356-img0003.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095] - Mass cytometry (surface staining) of human peripheral blood with anti-CD33 (WM53) 149Sm. Gated on singlets.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-WM53-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09095-img0002.jpg "Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095] - Mass cytometry (surface staining) of human peripheral blood with anti-CD33 (WM53) 149Sm. Gated on singlets.")
![Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095] - Analysis (surface staining) of human peripheral blood with anti-CD33 (WM53) purified antibody (low endotoxin), GAM-APC.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-WM53-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09095-img0001.jpg)
![Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-Immunohistochemistry-Paraffin-NBP2-32709-img0014.jpg "Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709] - Staining of human lung shows moderate positivity in macrophages.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-Immunohistochemistry-Paraffin-NBP2-32709-img0011.jpg)
![Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709] - Staining of human lymph node shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-Immunohistochemistry-Paraffin-NBP2-32709-img0012.jpg)
![Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709] - Staining of human placenta shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-Immunohistochemistry-Paraffin-NBP2-32709-img0013.jpg)
![Western Blot: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-278202415212472.jpg "Western Blot: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling Siglec-3/CD33 with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-29202410334035.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Flow cytometric analysis of TF-1 cells labeling Siglec-3/CD33. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32147, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-29202416453237.jpg)
![Western Blot: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: TF-1 cell lysate Lane 3: HL-60 cell lysate Lane 4: Jurkat cell lysate (negative) Lane 5: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32146) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32146_rabbit-siglec-3-cd33-mab-psh02-50-278202415212431.jpg "Western Blot: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: TF-1 cell lysate Lane 3: HL-60 cell lysate Lane 4: Jurkat cell lysate (negative) Lane 5: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32146) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling Siglec-3/CD33 with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32146_rabbit-siglec-3-cd33-mab-psh02-50-29202410484839.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Flow cytometric analysis of TF-1 cells labeling Siglec-3/CD33. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32146, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32146_rabbit-siglec-3-cd33-mab-psh02-50-29202416453211.jpg)