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Siglec-3/CD33 Antibody (2C6B7) - BSA Free

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Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of paraffin-embedded endometrium tissues using CD33 mouse mAb with DAB staining.
Flow Cytometry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of HepG2 cells using CD33 mouse mAb (green) and negative control (purple).
Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against human CD33 recombinant protein. (Expected MW is 49.2 kDa)
Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of paraffin-embedded esophageal cancer tissues using CD33 mouse mAb with DAB staining.
ELISA: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
2C6B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37388-0.025ml
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Siglec-3/CD33 Antibody (2C6B7) - BSA Free Summary

Immunogen
Purified recombinant fragment of human Siglec-3/CD33 (AA: 15-237) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD33
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
39.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Siglec-3/CD33 Antibody (2C6B7) - BSA Free

  • CD33 antigen (gp67)
  • CD33 antigen
  • CD33 molecule
  • CD33
  • FLJ00391
  • gp67
  • myeloid cell surface antigen CD33
  • p67
  • sialic acid binding Ig-like lectin 3
  • Sialic acid-binding Ig-like lectin 3
  • Siglec3
  • Siglec-3
  • SIGLEC3gp67

Background

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD33