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![Western Blot: TERF2IP Antibody [NB100-292] - Detection of Human RAP1 by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAP1 antibody NB100-292 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TERF2IP-Antibody-Western-Blot-NB100-292-img0019.jpg "<b>Biological Strategies Validation. </b>Western Blot: TERF2IP Antibody [NB100-292] - Detection of Human RAP1 by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAP1 antibody NB100-292 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: TERF2IP Antibody [NB100-292] - Sections of human lung carcinoma.~~ Antibody: Affinity purified rabbit anti-RAP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TERF2IP-Antibody-Immunohistochemistry-Paraffin-NB100-292-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: TERF2IP Antibody [NB100-292] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinitypurified rabbit anti-RAP1 antibody used for IP at 6 ug per reaction. RAP1 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-RAP1 antibody. For blotting immunoprecipitated RAP1, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/TERF2IP-Antibody-Immunoprecipitation-NB100-292-img0018.jpg)
![Western Blot: TERF2IP Antibody [NB100-56321] - Total protein from HeLa, SHSH-5Y, IMR-90 and MCF7 cells were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TERF2IP in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/TERF2IP-Antibody-Western-Blot-NB100-56321-img0004.jpg "Western Blot: TERF2IP Antibody [NB100-56321] - Total protein from HeLa, SHSH-5Y, IMR-90 and MCF7 cells were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TERF2IP in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: TERF2IP Antibody [NB100-56321] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-TERF2IP [NB100-56321] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A; Cat# NB100-690) was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TERF2IP-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56321-img0003.jpg)
![Western Blot: TERF2IP Antibody [NB100-56321] - TERF21P Antibody [NB100-56321] - 2 ug of this antibody was used to detect hRap1 in 293 cells. A protein band with an approximate molecular weight of 50 kDa was detected.](http://images.novusbio.com/fullsize/TERF21P-Antibody-Western-Blot-NB100-56321-img0002.jpg)
![Western Blot: TERF21P Antibody (78B356.1) [NB100-56526] - Western blot analysis of A431 (A), HEK293 (B) and HeLa (C) lysates (35ug per lane, RIPA buffer) using TERF21P antibody (NB100-56526) at 0.1ug/ml. Band observed at ~60kDa.](http://images.novusbio.com/fullsize/TERF21P-Antibody-78B356-1-Western-Blot-NB100-56526-img0003.jpg "Western Blot: TERF21P Antibody (78B356.1) [NB100-56526] - Western blot analysis of A431 (A), HEK293 (B) and HeLa (C) lysates (35ug per lane, RIPA buffer) using TERF21P antibody (NB100-56526) at 0.1ug/ml. Band observed at ~60kDa.")
![Western Blot: TERF2IP Antibody [NBP1-82433] - Analysis in control (vector only transfected HEK293T lysate) and TERF2IP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/TERF2IP-Antibody-Western-Blot-NBP1-82433-img0008.jpg "Western Blot: TERF2IP Antibody [NBP1-82433] - Analysis in control (vector only transfected HEK293T lysate) and TERF2IP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: TERF2IP Antibody [NBP1-82433] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.](http://images.novusbio.com/fullsize/TERF2IP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82433-img0007.jpg)
![Immunohistochemistry-Paraffin: TERF2IP Antibody [NBP1-82433] - Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.](http://images.novusbio.com/fullsize/TERF2IP-Antibody-Immunohistochemistry-Paraffin-NBP1-82433-img0001.jpg)
![Western Blot: TERF2IP Antibody [NBP1-82433] - Analysis in human cell line SCLC-21H.](http://images.novusbio.com/fullsize/TERF2IP-Antibody-Western-Blot-NBP1-82433-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TERF2IP Antibody (RAP1 4C8/1) [NB120-14404] - MEFs with anti-RAP1 antibody.](http://images.novusbio.com/fullsize/TERF2IP-Antibody-RAP1-4C8-1-Immunocytochemistry-Immunofluorescence-NB120-14404-img0002.jpg "Immunocytochemistry/Immunofluorescence: TERF2IP Antibody (RAP1 4C8/1) [NB120-14404] - MEFs with anti-RAP1 antibody.")
![Western Blot: RAP1 Overexpression Lysate (Adult Normal) [NBL1-16816] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAP1.](http://images.novusbio.com/fullsize/RAP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16816-img0002.jpg "Western Blot: RAP1 Overexpression Lysate (Adult Normal) [NBL1-16816] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAP1.")
![ELISA: Human TERF2IP - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40197] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40197_human-terf2ip-ready-to-use-elisa-kit-colorimetric-2110202415343917.png "ELISA: Human TERF2IP - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40197] - Standard Curve Reference")
![ELISA: Human TERF2IP ELISA Kit (Colorimetric) [NBP3-40198] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40198_human-terf2ip-elisa-kit-colorimetric-2110202415302962.png "ELISA: Human TERF2IP ELISA Kit (Colorimetric) [NBP3-40198] - Standard Curve Reference")