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![Western Blot: Optineurin Antibody [NBP1-84682] - Analysis in human cell lines A-431 and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-NBP1-84682-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Optineurin Antibody [NBP1-84682] - Analysis in human cell lines A-431 and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: Optineurin Antibody [NBP1-84682] - Analysis using Anti-OPTN antibody NBP1-84682 (A) shows similar pattern to independent antibody NBP1-84683 (B).](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-NBP1-84682-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: Optineurin Antibody [NBP1-84682] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining shown in green.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84682-img0012.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0016.jpg)
![Western Blot: Optineurin Antibody [NBP1-84682] - Effect of mitochondrial fission protein, Drp1 (FL) and mutant Tau on Optineurin expression in N2a (M) cells. Lanes: M. Ladder M. Ladder 1. N2a cells 2. N2a cells + mutant Tau 3. N2a cells + mutant tau + Drp1 full length + mutant Tau. This image was submitted via customer review.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-NBP1-84682-img0022.jpg)
![Immunohistochemistry-Frozen: Optineurin Antibody [NBP1-84682] - Image and review of Optineurin antibody on mouse retina (frozen sections) by Celia McKee, Duke University. 20X and 100X.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Frozen-NBP1-84682-img0006.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0013.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0014.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84682-img0015.jpg)
![Western Blot: Optineurin Antibody [NBP1-84683] - Analysis using Anti-OPTN antibody NBP1-84683 (A) shows similar pattern to independent antibody NBP1-84682 (B).](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-NBP1-84683-img0018.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Optineurin Antibody [NBP1-84683] - Analysis using Anti-OPTN antibody NBP1-84683 (A) shows similar pattern to independent antibody NBP1-84682 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Optineurin Antibody [NBP1-84683] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84683-img0007.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84683] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84683-img0019.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Optineurin Antibody [NBP1-84683] - Analysis in human cell lines A-431 and HEK293 using Anti-OPTN antibody. Corresponding OPTN RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-NBP1-84683-img0017.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84683] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84683-img0012.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84683] - Staining of human kidney shows weak cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84683-img0013.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84683] - Staining of human rectum shows moderate cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84683-img0014.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84683] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP1-84683-img0015.jpg)
![Western Blot: Optineurin Antibody [H00010133-B01P] - Analysis of OPTN expression in human placenta.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-H00010133-B01P-img0005.jpg "Western Blot: Optineurin Antibody [H00010133-B01P] - Analysis of OPTN expression in human placenta.")
![Immunocytochemistry/Immunofluorescence: Optineurin Antibody [H00010133-B01P] - Analysis of purified antibody to OPTN on HeLa cell. Antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunocytochemistry-Immunofluorescence-H00010133-B01P-img0004.jpg)
![Western Blot: Optineurin Antibody [H00010133-B01P] - Analysis of OPTN expression in transfected 293T cell line by OPTN polyclonal antibody. Lane 1: OPTN transfected lysate (63.47 kDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-H00010133-B01P-img0006.jpg)
![Simple Western: Optineurin Antibody [H00010133-B01P] - This antibody (1:25 dilution) was used to probe THP-1 macrophage lysate (concentrations shown) by Simple Western. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Simple-Western-H00010133-B01P-img0008.jpg)
![Western Blot: Optineurin Antibody [NBP3-13114] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Optineurin antibody (NBP3-13114) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-NBP3-13114-img0004.jpg "Western Blot: Optineurin Antibody [NBP3-13114] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Optineurin antibody (NBP3-13114) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Optineurin Antibody [NBP3-13114] - Optineurin antibody detects Optineurin protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Optineurin protein stained by Optineurin antibody (NBP3-13114) diluted at 1:500.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13114-img0002.jpg)
![Immunohistochemistry-Paraffin: Optineurin Antibody [NBP3-13114] - Optineurin antibody detects Optineurin protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Optineurin antibody (NBP3-13114) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunohistochemistry-Paraffin-NBP3-13114-img0003.jpg)
![Western Blot: Optineurin Antibody [NBP3-13114] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Optineurin antibody (NBP3-13114) diluted at 1:2000.](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-NBP3-13114-img0001.jpg)
![Western Blot: Optineurin Antibody (S01-2C5) [NBP3-19900] - Western blot detection of Optineurin in Hela, A549, HL-60, U2OS, U87-MG cell lysates using NBP3-19900 (1:1000 diluted). Predicted band size: 66kDa. Observed band size: 75kDa.](http://images.novusbio.com/fullsize/Optineurin-Antibody-S01-2C5-Western-Blot-NBP3-19900-img0002.jpg "Western Blot: Optineurin Antibody (S01-2C5) [NBP3-19900] - Western blot detection of Optineurin in Hela, A549, HL-60, U2OS, U87-MG cell lysates using NBP3-19900 (1:1000 diluted). Predicted band size: 66kDa. Observed band size: 75kDa.")
![Immunohistochemistry-Paraffin: Optineurin Antibody (S01-2C5) [NBP3-19900] - Immunohistochemistry of Optineurin in paraffin-embedded Human Cholangiocarcinoma using NBP3-19900 at dilution 1/50](http://images.novusbio.com/fullsize/Optineurin-Antibody-S01-2C5-Immunohistochemistry-Paraffin-NBP3-19900-img0001.jpg)
![Western Blot: Optineurin Antibody [NBP3-00298] - Anti-Optineurin rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lane C: Hela Whole Cell Lysate. Lane D: NCI-H1299 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 66 kDa. Observed band size: 75 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Optineurin-Antibody-Western-Blot-NBP3-00298-img0004.jpg "Western Blot: Optineurin Antibody [NBP3-00298] - Anti-Optineurin rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lane C: Hela Whole Cell Lysate. Lane D: NCI-H1299 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 66 kDa. Observed band size: 75 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Optineurin Antibody [NBP3-00298] - Optineurin was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-Optineurin rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: Anti-Optineurin rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 66 kDa. Observed band size: 75 kDa](http://images.novusbio.com/fullsize/Optineurin-Antibody-Immunoprecipitation-NBP3-00298-img0003.jpg)
![Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBP2-11186] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.](http://images.novusbio.com/fullsize/Optineurin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11186-img0001.jpg "Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBP2-11186] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.")
![Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBL1-13949] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.](http://images.novusbio.com/fullsize/Optineurin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13949-img0002.jpg "Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBL1-13949] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.")
![Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBP2-11185] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.](http://images.novusbio.com/fullsize/Optineurin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11185-img0001.jpg "Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBP2-11185] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.")
![Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBP2-11265] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.](http://images.novusbio.com/fullsize/Optineurin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11265-img0001.jpg "Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBP2-11265] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.")
![Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBP2-10996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.](http://images.novusbio.com/fullsize/Optineurin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10996-img0001.jpg "Western Blot: Optineurin Overexpression Lysate (Adult Normal) [NBP2-10996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Optineurin.")