| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-294-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4006-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08877 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-74027-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54478-100ug | |||
![Western Blot: Aurora B Antibody - BSA Free [NB100-294] - Whole extract of PC-12 cells were separated on SDS-PAGE and probed with Anti-Aurora B antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. Negative Control](http://images.novusbio.com/fullsize/Aurora-B-Antibody---BSA-Free-Western-Blot-NB100-294-img0002.jpg "Western Blot: Aurora B Antibody - BSA Free [NB100-294] - Whole extract of PC-12 cells were separated on SDS-PAGE and probed with Anti-Aurora B antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. Negative Control")
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294] - HeLa human cervix epitheloid carcinoma cells were fixed and permeabilized with 4% paraformaldehyde followed by cold methanol. Fixed cells were stained with 1:50 Anti-Aurora B antibody produced in rabbit. The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat.](http://images.novusbio.com/fullsize/Aurora-B-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-294-img0001.jpg)
![Western Blot: Aurora B Antibody (3F11) [NBP2-50039] - Analysis of different cell lysates and recombinant protein solutions using mouse mAb to Aurora B. Left: cells were treated with 100ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: Blot of purified full length recombinant human aurora A, B and C were probed with NBP2-50039. The antibody binds specifically only to aurora B and not to the closely related aurora A and C.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-3F11-Western-Blot-NBP2-50039-img0006.jpg "Western Blot: Aurora B Antibody (3F11) [NBP2-50039] - Analysis of different cell lysates and recombinant protein solutions using mouse mAb to Aurora B. Left: cells were treated with 100ng/mL of nocodazol, a microtubule depolymerizing agent which induces cells to halt at G2/M phase. [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] equine NBL6 cells, and [5] mouse KR158 cells. Right: Blot of purified full length recombinant human aurora A, B and C were probed with NBP2-50039. The antibody binds specifically only to aurora B and not to the closely related aurora A and C.")
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (3F11) [NBP2-50039] - High magnification confocal immunofluorescence of HeLa cells stained with NBP2-50039, diluted 1:1,000 in green and counterstained with antibody to vimentin at 1:1,000 dilution in red. Blue is the DNA stain DAPI revealing the nuclei. The MCA-3F11 reveals aurora B localized in midbodies, midzones of dividing cells and also in the nuclei or some cells.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-3F11-Immunocytochemistry-Immunofluorescence-NBP2-50039-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (3F11) [NBP2-50039] - HeLa cell cultures were stained with NBP2-50039 (green). Aurora B stains midzones in anaphase and midbodies between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-3F11-Immunocytochemistry-Immunofluorescence-NBP2-50039-img0003.jpg)
![Western Blot: Aurora B Antibody (6G2) [NBP2-50054] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with NBP2-50054. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. NBP2-50054 binds strongly to Aurora B at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with NBP2-50054.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-6G2-Western-Blot-NBP2-50054-img0004.jpg "Western Blot: Aurora B Antibody (6G2) [NBP2-50054] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with NBP2-50054. Nocodazole is a microtubule polymerization inhibitor which induces cells to halt at the G2/M phase and also induces Aurora B expression. NBP2-50054 binds strongly to Aurora B at 38 kDa. Right: Blots of recombinant human Aurora A, B and C were probed with NBP2-50054.")
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054] - Analysis of HeLa cells stained with mouse mAb to aurora B kinase, NBP2-50054, dilution 1:1,000 in red, and costained with chicken pAb to vimentin, dilution 1:10,000 in green. Blue is DAPI staining of nuclear DNA. NBP2-50054 antibody produces strong staining associated with chromosomes in prophase (A), the centromere in prometaphase and metaphase (B), the central mitotic spindle in anaphase (C), and midbodies between the two daughter cells during telophase and beyond (D). The vimentin antibody stains the intermediate filament network in these cells.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-6G2-Immunocytochemistry-Immunofluorescence-NBP2-50054-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (6G2) [NBP2-50054] - HeLa cell cultures were stained with NBP2-50054 (green). Localization of Aurora B is cell cycle phase dependent. First, Aurora B staining was seen in chromosome arm in cells in prophase (green small dots). During anaphase, Aurora B localized in midzone and then concentrated in midbodies between the two daughter cells during telophase. It is therefore a useful marker of midbodies and dividing cells. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, red). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-6G2-Immunocytochemistry-Immunofluorescence-NBP2-50054-img0003.jpg)
![Western Blot: Aurora B Antibody [NB500-185] - Detection of Human Aurora B by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells treated with nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Aurora B antibody NB500-185 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-Western-Blot-NB500-185-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: Aurora B Antibody [NB500-185] - Detection of Human Aurora B by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa cells treated with nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Aurora B antibody NB500-185 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Aurora B Antibody [NB500-185] - Asynchronous HeLa cells. Antibody used at 0.5 ug/ml in A and at 2 ug/ml in B. Detection : Secondary antibodies were anti-Rabbit IgG conjugated to FITC in A and conjugated to Texas Red in B.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-Immunocytochemistry-Immunofluorescence-NB500-185-img0004.jpg)
![Immunoprecipitation: Aurora B Antibody [NB500-185] - Detection of human Aurora B by western blot of immunoprecipitation. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from nocodazole treated HeLa cells. Antibody: Affinity purified rabbit anti-Aurora B antibody NB500-185 (lot NB500-185-2) used at 0.1 ug/ml. Aurora B was also immunoprecipitated by a previous lot of this antibody (lot NB500-185-1). Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-Immunoprecipitation-NB500-185-img0007.jpg)
![Immunoprecipitation: Aurora B Antibody (4E5) [NBP3-26486] - Immunoprecipitating Aurora B in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26486 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26486 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-26220241322158.jpg "Immunoprecipitation: Aurora B Antibody (4E5) [NBP3-26486] - Immunoprecipitating Aurora B in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26486 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26486 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Immunohistochemistry: Aurora B Antibody (4E5) [NBP3-26486] - Image of Aurora B Antibody (4E5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-262202415253321.jpg)
![Immunohistochemistry: Aurora B Antibody (4E5) [NBP3-26486] - Image of Aurora B Antibody (4E5) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-2722024827492.jpg)
![Western Blot: Aurora B Antibody (4E5) [NBP3-26486] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate. All lanes: Aurora B Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40, 36, 17, 35 kDa Observed band size: 40 kDa](http://images.novusbio.com/fullsize/nbp3-26486_rabbit-aurora-b-mab-4e5-282202411442635.jpg)
![Western Blot: Aurora B Antibody (RM278) [NBP2-61493] - Western Blot of HeLa cells nontreated (-) or treated (+) with Nocodazole (NOC), using NBP2-61493 at a 1:1000 dilution](http://images.novusbio.com/fullsize/Aurora-B-Antibody-RM278-Western-Blot-NBP2-61493-img0004.jpg "Western Blot: Aurora B Antibody (RM278) [NBP2-61493] - Western Blot of HeLa cells nontreated (-) or treated (+) with Nocodazole (NOC), using NBP2-61493 at a 1:1000 dilution")
![Immunohistochemistry-Paraffin: Aurora B Antibody (RM278) [NBP2-61493] - Immunohistochemical staining of formalin fixed and paraffin embedded Human tonsil tissue sections using NBP2-61493 at a 1:500 dilution](http://images.novusbio.com/fullsize/Aurora-B-Antibody-RM278-Immunohistochemistry-Paraffin-NBP2-61493-img0003.jpg)
![Western Blot: Aurora B Antibody (1N9V5) [NBP3-15762] - Western blot analysis of extracts of various cell lines, using Aurora B antibody (NBP3-15762) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-1N9V5-Western-Blot-NBP3-15762-img0002.jpg "Western Blot: Aurora B Antibody (1N9V5) [NBP3-15762] - Western blot analysis of extracts of various cell lines, using Aurora B antibody (NBP3-15762) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Aurora B Antibody (1N9V5) [NBP3-15762] - Immunofluorescence analysis of C6 cells using Aurora B antibody (NBP3-15762). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Aurora-B-Antibody-1N9V5-Immunocytochemistry-Immunofluorescence-NBP3-15762-img0001.jpg)
![Immunohistochemistry-Paraffin: Aurora B Antibody (rAURKB/1592) - Azide and BSA Free [NBP3-08877] - Formalin-fixed, paraffin-embedded human kidney stained with Aurora B Recombinant Mouse Monoclonal Antibody (rAURKB/1592).](http://images.novusbio.com/fullsize/Aurora-B-Antibody-rAURKB-1592-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08877-img0002.jpg "Immunohistochemistry-Paraffin: Aurora B Antibody (rAURKB/1592) - Azide and BSA Free [NBP3-08877] - Formalin-fixed, paraffin-embedded human kidney stained with Aurora B Recombinant Mouse Monoclonal Antibody (rAURKB/1592).")
![Immunohistochemistry-Paraffin: Aurora B Antibody (rAURKB/1592) - Azide and BSA Free [NBP3-08877] - Formalin-fixed, paraffin-embedded human kidney stained with Aurora B Recombinant Mouse Monoclonal Antibody (rAURKB/1592).](http://images.novusbio.com/fullsize/Aurora-B-Antibody-rAURKB-1592-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08877-img0001.jpg)
![Immunohistochemistry-Paraffin: Aurora B Antibody (rAURKB/1592) - Azide and BSA Free [NBP3-08877] - Formalin-fixed, paraffin-embedded human placenta stained with Aurora B Recombinant Mouse Monoclonal Antibody (rAURKB/1592).](http://images.novusbio.com/fullsize/Aurora-B-Antibody-rAURKB-1592-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08877-img0003.jpg)
![Immunohistochemistry-Paraffin: Aurora B Antibody (rAURKB/1592) [NBP3-07553] - Formalin-fixed, paraffin-embedded human placenta stained with Aurora B Recombinant Mouse Monoclonal Antibody (rAURKB/1592).](http://images.novusbio.com/fullsize/Aurora-B-Antibody-rAURKB-1592-Immunohistochemistry-Paraffin-NBP3-07553-img0003.jpg "Immunohistochemistry-Paraffin: Aurora B Antibody (rAURKB/1592) [NBP3-07553] - Formalin-fixed, paraffin-embedded human placenta stained with Aurora B Recombinant Mouse Monoclonal Antibody (rAURKB/1592).")
![Immunohistochemistry-Paraffin: Aurora B Antibody (rAURKB/1592) [NBP3-07553] - Formalin-fixed, paraffin-embedded human kidney stained with Aurora B Recombinant Mouse Monoclonal Antibody (rAURKB/1592).](http://images.novusbio.com/fullsize/Aurora-B-Antibody-rAURKB-1592-Immunohistochemistry-Paraffin-NBP3-07553-img0001.jpg)
![Immunohistochemistry-Paraffin: Aurora B Antibody (rAURKB/1592) [NBP3-07553] - Formalin-fixed, paraffin-embedded human kidney stained with Aurora B Recombinant Mouse Monoclonal Antibody (rAURKB/1592).](http://images.novusbio.com/fullsize/Aurora-B-Antibody-rAURKB-1592-Immunohistochemistry-Paraffin-NBP3-07553-img0002.jpg)
![Western Blot: Aurora B Antibody (2B1) [NBP1-74027] - The recombinant proteins (50ng) were resolved by SDSPAGE,transferred to PVDF membrane and probed with antihumanAurora kinase B antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibodyconjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human Aurora kinase ALane 2.: Recombinant Human Aurora kinase B](http://images.novusbio.com/fullsize/Aurora-B-Antibody-2B1-Western-Blot-NBP1-74027-img0005.jpg "Western Blot: Aurora B Antibody (2B1) [NBP1-74027] - The recombinant proteins (50ng) were resolved by SDSPAGE,transferred to PVDF membrane and probed with antihumanAurora kinase B antibody (1:1000). Proteins werevisualized using a goat anti-mouse secondary antibodyconjugated to HRP and an ECL detection system.Lane 1.: Recombinant Human Aurora kinase ALane 2.: Recombinant Human Aurora kinase B")
![Flow Cytometry: Aurora B Antibody (2B1) [NBP1-74027] - Analysis of Aurora B in LNCap cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/Aurora-B-Antibody-2B1-Flow-Cytometry-NBP1-74027-img0006.jpg)
![Western Blot: Aurora B Antibody (AURKB/1521) [NBP2-53267] - Western Blot Analysis (A) Recombinant Protein (B) Human Liver Lysate Using Aurora B Monoclonal Antibody (AURKB/1521).](http://images.novusbio.com/fullsize/Aurora-B-Antibody-AURKB-1521-Western-Blot-NBP2-53267-img0001.jpg "Western Blot: Aurora B Antibody (AURKB/1521) [NBP2-53267] - Western Blot Analysis (A) Recombinant Protein (B) Human Liver Lysate Using Aurora B Monoclonal Antibody (AURKB/1521).")