| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76757-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52459-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79918 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52460-0.025mg | |||
![Western Blot: Emerin Antibody [NBP1-87692] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Emerin-Antibody-Western-Blot-NBP1-87692-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Emerin Antibody [NBP1-87692] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Emerin Antibody [NBP1-87692] - Staining of human cell line U-251 MG shows localization to nuclear membrane & endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Emerin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87692-img0010.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692] - Staining of human skeletal muscle shows moderate nuclear membrane positivity in myocytes.](http://images.novusbio.com/fullsize/Emerin-Antibody-Immunohistochemistry-Paraffin-NBP1-87692-img0006.jpg)
![Western Blot: Emerin Antibody [NBP1-87692] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/Emerin-Antibody-Western-Blot-NBP1-87692-img0011.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692] -Staining of human heart muscle shows strong positivity in nuclear membrane in cardiomyocytes.](http://images.novusbio.com/fullsize/antibody/nbp1-87692_rabbit-polyclonal-emerin-antibody-immunohistochemistry-paraffin-21122023105440..jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692] -Staining of human fallopian tube shows moderate positivity in nuclear membrane in glandular cells and smooth muscle cells with additional weak cytoplasmic positivity.](http://images.novusbio.com/fullsize/antibody/nbp1-87692_rabbit-polyclonal-emerin-antibody-immunohistochemistry-paraffin-21122023105539..jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692]- Staining of human testis shows moderate positivity in nuclear membrane in cells in seminiferous ducts and leydig cells with weak cytoplasmic positivity.](http://images.novusbio.com/fullsize/antibody/nbp1-87692_rabbit-polyclonal-emerin-antibody-immunohistochemistry-paraffin-21122023105658..jpg)
![Western Blot: Emerin Antibody (CL0201) [NBP2-52876] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-EMD antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0201-Western-Blot-NBP2-52876-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Emerin Antibody (CL0201) [NBP2-52876] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-EMD antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Western Blot: Emerin Antibody (CL0201) [NBP2-52876] - Lane 1: Marker [kDa] Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0201-Western-Blot-NBP2-52876-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0201) [NBP2-52876] - Staining of PC-3 cells using the Anti-EMD monoclonal antibody, showing specific staining in the nuclear membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0201-Immunocytochemistry-Immunofluorescence-NBP2-52876-img0008.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876] - Staining of human stomach shows strong positivity in the nuclear membrane of glandular cells.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0201-Immunohistochemistry-Paraffin-NBP2-52876-img0004.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876] - Staining of human fallopian tube shows strong positivity in the nuclear membrane of glandular cells.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0201-Immunohistochemistry-Paraffin-NBP2-52876-img0001.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876] - Staining of human kidney shows strong immunoreactivity in the nuclear membrane in renal glomeruli and tubuli.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0201-Immunohistochemistry-Paraffin-NBP2-52876-img0002.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (CL0201) [NBP2-52876] - Staining of human placenta shows strong immunoreactivity in the nuclear membrane in the trophoblast layer.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0201-Immunohistochemistry-Paraffin-NBP2-52876-img0003.jpg)
![Western Blot: Emerin Antibody (CL0203) [NBP2-52877] - Analysis of extracts from RT-4 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-EMD monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Western-Blot-NBP2-52877-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Emerin Antibody (CL0203) [NBP2-52877] - Analysis of extracts from RT-4 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-EMD monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in U2OS cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunocytochemistry-Immunofluorescence-NBP2-52877-img0005.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human testis shows nuclear membrane immunoreactivity.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunohistochemistry-Paraffin-NBP2-52877-img0010.jpg)
![Western Blot: Emerin Antibody (CL0203) [NBP2-52877] - Lane 1: Marker [kDa], Lane 2: RT-4](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Western-Blot-NBP2-52877-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in A431 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunocytochemistry-Immunofluorescence-NBP2-52877-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in HeLa cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunocytochemistry-Immunofluorescence-NBP2-52877-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in MCF7 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunocytochemistry-Immunofluorescence-NBP2-52877-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in U251 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunocytochemistry-Immunofluorescence-NBP2-52877-img0004.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human kidney shows nuclear membrane positivity in renal tubules.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunohistochemistry-Paraffin-NBP2-52877-img0006.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human placenta shows strong nuclear membrane positivity in the trophoblast.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunohistochemistry-Paraffin-NBP2-52877-img0007.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human prostate shows strong nuclear membrane immunoreactivityy in glandular cells.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunohistochemistry-Paraffin-NBP2-52877-img0008.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human skin shows strong nuclear membrane positivity in the epidermis cells.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunohistochemistry-Paraffin-NBP2-52877-img0009.jpg)
![Western Blot: Emerin Antibody [NBP1-76757] - Human skeletal muscle tissue lysate with Emerin antibody at (A) 0.5 and (B) 1 ug/ml.](http://images.novusbio.com/fullsize/Emerin-Antibody-Western-Blot-NBP1-76757-img0002.jpg "Western Blot: Emerin Antibody [NBP1-76757] - Human skeletal muscle tissue lysate with Emerin antibody at (A) 0.5 and (B) 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Emerin Antibody [NBP1-76757] - Immunofluorescence of Emerin in Human Skeletal Muscle cells with Emerin antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/Emerin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76757-img0003.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-76757] - Human skeletal muscle tissue with emerin antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Emerin-Antibody-Immunohistochemistry-Paraffin-NBP1-76757-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Emerin Antibody - BSA Free [NBP1-76757] - Immunofluorescence of Emerin in Human Skeletal Muscle cells with Emerin antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76757_rabbit-polyclonal-emerin-antibody-271120242013167.jpg)
![Immunohistochemistry: Emerin Antibody - BSA Free [NBP1-76757] - Immunohistochemistry of emerin in human skeletal muscle tissue with emerin antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76757_rabbit-polyclonal-emerin-antibody-2711202419451214.jpg)
![Immunohistochemistry: Emerin Antibody - BSA Free [NBP1-76757] - Immunohistochemistry of emerin in human skeletal muscle tissue with emerin antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76757_rabbit-polyclonal-emerin-antibody-2711202420324850.jpg)
![Immunocytochemistry/ Immunofluorescence: Emerin Antibody - BSA Free [NBP1-76757] - Immunofluorescence of Emerin in Human Skeletal Muscle cells with Emerin antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76757_rabbit-polyclonal-emerin-antibody-271120242032623.jpg)
![Western Blot: Emerin Antibody (8F5A8) [NBP2-52459] - Analysis using EMD mAb against human EMD (AA: 1-222) recombinant protein. (Expected MW is 51.1 kDa)](http://images.novusbio.com/fullsize/Emerin-Antibody-8F5A8-Western-Blot-NBP2-52459-img0005.jpg "Western Blot: Emerin Antibody (8F5A8) [NBP2-52459] - Analysis using EMD mAb against human EMD (AA: 1-222) recombinant protein. (Expected MW is 51.1 kDa)")
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (8F5A8) [NBP2-52459] - Analysis of Hela cells using EMD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Emerin-Antibody-8F5A8-Immunofluorescence-NBP2-52459-img0003.jpg)
![Immunohistochemistry: Emerin Antibody (8F5A8) [NBP2-52459] - Analysis of paraffin-embedded ovarian cancer tissues using EMD mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Emerin-Antibody-8F5A8-Immunohistochemistry-NBP2-52459-img0004.jpg)
![Flow Cytometry: Emerin Antibody (8F5A8) [NBP2-52459] - Analysis of K562 cells using EMD mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Emerin-Antibody-8F5A8-Flow-Cytometry-NBP2-52459-img0002.jpg)
![Western Blot: Emerin Antibody (8F5A8) [NBP2-52459] - Analysis using EMD mAb against HEK293 (1) and EMD (AA: 1-222)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Emerin-Antibody-8F5A8-Western-Blot-NBP2-52459-img0006.jpg)
![Flow Cytometry: Emerin Antibody (8F5A8) [NBP2-52459] - Analysis of A549 cells using EMD mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Emerin-Antibody-8F5A8-Flow-Cytometry-NBP2-52459-img0001.jpg)
![ELISA: Emerin Antibody (8F5A8) [NBP2-52459] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Emerin-Antibody-8F5A8-ELISA-NBP2-52459-img0008.jpg)
![Western Blot: Emerin Antibody (EMD/2167) - Azide and BSA Free [NBP2-79918] - Western Blot Analysis of human HeLa cell lysate using Emerin Mouse Monoclonal Antibody (EMD/2167).](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Azide-and-BSA-Free-Western-Blot-NBP2-79918-img0004.jpg "Western Blot: Emerin Antibody (EMD/2167) - Azide and BSA Free [NBP2-79918] - Western Blot Analysis of human HeLa cell lysate using Emerin Mouse Monoclonal Antibody (EMD/2167).")
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (EMD/2167) - Azide and BSA Free [NBP2-79918] - Immunofluorescence Analysis of Human HeLa cells labeling Emerin with Emerin Mouse Monoclonal Antibody (EMD/2167) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-79918-img0005.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) - Azide and BSA Free [NBP2-79918] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2167).](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79918-img0003.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) - Azide and BSA Free [NBP2-79918] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2167).](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79918-img0001.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) - Azide and BSA Free [NBP2-79918] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2167).](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79918-img0002.jpg)
![Protein Array: Emerin Antibody (EMD/2167) - Azide and BSA Free [NBP2-79918] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Azide-and-BSA-Free-Protein-Array-NBP2-79918-img0006.jpg)
![Western Blot: Emerin Antibody (EMD/2167) [NBP2-79784] - Western Blot Analysis of human HeLa cell lysate using Emerin Antibody (EMD/2167).](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Western-Blot-NBP2-79784-img0006.jpg "Western Blot: Emerin Antibody (EMD/2167) [NBP2-79784] - Western Blot Analysis of human HeLa cell lysate using Emerin Antibody (EMD/2167).")
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (EMD/2167) [NBP2-79784] - Immunofluorescence Analysis of Human HeLa cells labeling Emerin with Emerin Antibody (EMD/2167) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red)](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Immunocytochemistry-Immunofluorescence-NBP2-79784-img0001.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Emerin Antibody (EMD/2167).](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Immunohistochemistry-Paraffin-NBP2-79784-img0004.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Emerin Antibody (EMD/2167).](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Immunohistochemistry-Paraffin-NBP2-79784-img0002.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Emerin Antibody (EMD/2167).](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Immunohistochemistry-Paraffin-NBP2-79784-img0003.jpg)
![Protein Array: Emerin Antibody (EMD/2167) [NBP2-79784] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Emerin Antibody (EMD/2167) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Protein-Array-NBP2-79784-img0005.jpg)
![Western Blot: Emerin Antibody (8F5G2) [NBP2-52460] - Analysis using EMD mAb against human EMD (AA: 1-222) recombinant protein. (Expected MW is 51.1 kDa)](http://images.novusbio.com/fullsize/Emerin-Antibody-8F5G2-Western-Blot-NBP2-52460-img0005.jpg "Western Blot: Emerin Antibody (8F5G2) [NBP2-52460] - Analysis using EMD mAb against human EMD (AA: 1-222) recombinant protein. (Expected MW is 51.1 kDa)")
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (8F5G2) [NBP2-52460] - Analysis of Hela cells using EMD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Emerin-Antibody-8F5G2-Immunofluorescence-NBP2-52460-img0004.jpg)
![Flow Cytometry: Emerin Antibody (8F5G2) [NBP2-52460] - Analysis of A549 cells using EMD mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Emerin-Antibody-8F5G2-Flow-Cytometry-NBP2-52460-img0002.jpg)
![Western Blot: Emerin Antibody (8F5G2) [NBP2-52460] - Analysis using EMD mAb against HEK293 (1) and EMD (AA: 1-222)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Emerin-Antibody-8F5G2-Western-Blot-NBP2-52460-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (8F5G2) [NBP2-52460] - Immunofluorescence analysis of A549 cells using EMD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Emerin-Antibody-8F5G2-Immunofluorescence-NBP2-52460-img0003.jpg)
![Flow Cytometry: Emerin Antibody (8F5G2) [NBP2-52460] - Analysis of K562 cells using EMD mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Emerin-Antibody-8F5G2-Flow-Cytometry-NBP2-52460-img0001.jpg)
![ELISA: Emerin Antibody (8F5G2) [NBP2-52460] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Emerin-Antibody-8F5G2-ELISA-NBP2-52460-img0007.jpg)
![Western Blot: Emerin Antibody [NBP2-16326] - Wild-type (WT) and Emerin knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Emerin antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Emerin-Antibody-Western-Blot-NBP2-16326-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Emerin Antibody [NBP2-16326] - Wild-type (WT) and Emerin knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Emerin antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Emerin Antibody [NBP2-16326] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Emerin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16326-img0003.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody [NBP2-16326] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/Emerin-Antibody-Immunohistochemistry-Paraffin-NBP2-16326-img0002.jpg)
![Western Blot: Emerin Antibody [NBP2-16326] - Sample (30 ug of whole cell lysate) A: HeLa nucleus 12% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/Emerin-Antibody-Western-Blot-NBP2-16326-img0001.jpg)
![Western Blot: Emerin Antibody (10Y5K9) [NBP3-16351] - Western blot analysis of extracts of various cell lines, using Emerin Rabbit mAb (NBP3-16351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Emerin-Antibody-10Y5K9-Western-Blot-NBP3-16351-img0004.jpg "Western Blot: Emerin Antibody (10Y5K9) [NBP3-16351] - Western blot analysis of extracts of various cell lines, using Emerin Rabbit mAb (NBP3-16351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (10Y5K9) [NBP3-16351] - Immunofluorescence analysis of NIH-3T3 cells using Emerin Rabbit mAb (NBP3-16351) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Emerin-Antibody-10Y5K9-Immunocytochemistry-Immunofluorescence-NBP3-16351-img0003.jpg)
![Immunohistochemistry-Paraffin: Emerin Antibody (10Y5K9) [NBP3-16351] - Immunohistochemistry of paraffin-embedded human colon using Emerin Rabbit mAb (NBP3-16351) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Emerin-Antibody-10Y5K9-Immunohistochemistry-Paraffin-NBP3-16351-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (10Y5K9) [NBP3-16351] - Immunofluorescence analysis of HeLa cells using Emerin Rabbit mAb (NBP3-16351) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Emerin-Antibody-10Y5K9-Immunocytochemistry-Immunofluorescence-NBP3-16351-img0002.jpg)
![Immunohistochemistry: Emerin Antibody (PSH01-86) [NBP3-32308] - Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-Emerin antibody (NBP3-32308) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32308_rabbit-emerin-mab-psh01-86-298202414161876.jpg "Immunohistochemistry: Emerin Antibody (PSH01-86) [NBP3-32308] - Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-Emerin antibody (NBP3-32308) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Emerin Antibody (PSH01-86) [NBP3-32308] - Immunocytochemistry analysis of HeLa cells labeling Emerin with Rabbit anti-Emerin antibody (NBP3-32308) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Emerin antibody (NBP3-32308) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32308_rabbit-emerin-mab-psh01-86-29202410484896.jpg)
![Flow Cytometry: Emerin Antibody (PSH01-86) [NBP3-32308] - Flow cytometric analysis of HeLa cells labeling Emerin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32308, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32308_rabbit-emerin-mab-psh01-86-29202416372138.jpg)
![Western Blot: Emerin Antibody (PSH01-86) [NBP3-32308] - Western blot analysis of Emerin on different lysates with Rabbit anti-Emerin antibody (NBP3-32308) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: A549 cell lysate Lane 6: THP-1 cell lysate (negative) Lane 7: K-562 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 29 kDa Observed band size: 35 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32308_rabbit-emerin-mab-psh01-86-3920248453518.jpg)
![Western Blot: Emerin Antibody (PSH01-85) [NBP3-32307] - Western blot analysis of Emerin on different lysates with Rabbit anti-Emerin antibody (NBP3-32307) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: A549 cell lysate Lane 6: K-562 cell lysate Lane 7: Jurkat cell lysate Lane 8: Daudi cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 35 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32307) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32307_rabbit-emerin-mab-psh01-85-278202416285216.jpg "Western Blot: Emerin Antibody (PSH01-85) [NBP3-32307] - Western blot analysis of Emerin on different lysates with Rabbit anti-Emerin antibody (NBP3-32307) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: A549 cell lysate Lane 6: K-562 cell lysate Lane 7: Jurkat cell lysate Lane 8: Daudi cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 35 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32307) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Emerin Antibody (PSH01-85) [NBP3-32307] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Emerin antibody (NBP3-32307) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32307) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32307_rabbit-emerin-mab-psh01-85-29820241435218.jpg)
![Immunocytochemistry/ Immunofluorescence: Emerin Antibody (PSH01-85) [NBP3-32307] - Immunocytochemistry analysis of HeLa cells labeling Emerin with Rabbit anti-Emerin antibody (NBP3-32307) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Emerin antibody (NBP3-32307) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32307_rabbit-emerin-mab-psh01-85-2920241114834.jpg)
![Flow Cytometry: Emerin Antibody (PSH01-85) [NBP3-32307] - Flow cytometric analysis of HeLa cells labeling Emerin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32307, 1 /1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32307_rabbit-emerin-mab-psh01-85-29202416372130.jpg)