![Western Blot: Emerin Antibody (EMD/2167) [NBP2-79784] - Western Blot Analysis of human HeLa cell lysate using Emerin Antibody (EMD/2167).](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Western-Blot-NBP2-79784-img0006.jpg "Western Blot: Emerin Antibody (EMD/2167) [NBP2-79784] - Western Blot Analysis of human HeLa cell lysate using Emerin Antibody (EMD/2167).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, MA |
| Clone | EMD/2167 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79918) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human Emerin protein fragment (around aa 56-167) (exact sequence is proprietary) (Uniprot: P50402) |
| Localization | Nuclear Membrane |
| Marker | Papillary Thyroid Carcinoma and EDMD Marker |
| Specificity | Emerin is a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreifuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Reportedly, lack of Emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherents in the heart. Identification of nuclear membrane irregularities with anti-emerin antibody has been reported useful in diagnosing papillary thyroid carcinoma. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | EMD |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Emerin Antibody (EMD/2167) [NBP2-79784] - Immunofluorescence Analysis of Human HeLa cells labeling Emerin with Emerin Antibody (EMD/2167) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red)](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Immunocytochemistry-Immunofluorescence-NBP2-79784-img0001.jpg "Immunocytochemistry/Immunofluorescence: Emerin Antibody (EMD/2167) [NBP2-79784] - Immunofluorescence Analysis of Human HeLa cells labeling Emerin with Emerin Antibody (EMD/2167) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red)")
![Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Emerin Antibody (EMD/2167).](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Immunohistochemistry-Paraffin-NBP2-79784-img0004.jpg "Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Emerin Antibody (EMD/2167).")
![Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Emerin Antibody (EMD/2167).](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Immunohistochemistry-Paraffin-NBP2-79784-img0002.jpg "Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Emerin Antibody (EMD/2167).")
![Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Emerin Antibody (EMD/2167).](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Immunohistochemistry-Paraffin-NBP2-79784-img0003.jpg "Immunohistochemistry-Paraffin: Emerin Antibody (EMD/2167) [NBP2-79784] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Emerin Antibody (EMD/2167).")
![Protein Array: Emerin Antibody (EMD/2167) [NBP2-79784] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Emerin Antibody (EMD/2167) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Emerin-Antibody-EMD-2167-Protein-Array-NBP2-79784-img0005.jpg "Protein Array: Emerin Antibody (EMD/2167) [NBP2-79784] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Emerin Antibody (EMD/2167) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.")
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