![Western Blot: Emerin Antibody (CL0203) [NBP2-52877] - Analysis of extracts from RT-4 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-EMD monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Western-Blot-NBP2-52877-img0012.jpg "Western Blot: Emerin Antibody (CL0203) [NBP2-52877] - Analysis of extracts from RT-4 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-EMD monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KD |
| Clone | CL0203 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to amino acids: ASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSACQSITHY |
| Epitope | QSKGYNDDYY |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | EMD |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Protein A purified |
![Immunohistochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunohistochemistry_20892.jpg)
![Immunocytochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunocytochemistry__Immunofluorescence_21143.jpg)
![Western Blot EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Western_Blot_19925.jpg)
| Publication using NBP2-52877 | Applications | Species |
|---|---|---|
| Neggers JE, Paolella BR, Asfaw A et al. Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q Cell reports 2020-12-15 [PMID: 33326793] (ICC/IF, Human) | ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Emerin Antibody (NBP2-52877)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | EMD |
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in U2OS cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunocytochemistry-Immunofluorescence-NBP2-52877-img0005.jpg "Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in U2OS cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human testis shows nuclear membrane immunoreactivity.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunohistochemistry-Paraffin-NBP2-52877-img0010.jpg "Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human testis shows nuclear membrane immunoreactivity.")
![Western Blot: Emerin Antibody (CL0203) [NBP2-52877] - Lane 1: Marker [kDa], Lane 2: RT-4](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Western-Blot-NBP2-52877-img0011.jpg "Western Blot: Emerin Antibody (CL0203) [NBP2-52877] - Lane 1: Marker [kDa], Lane 2: RT-4")
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in A431 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunocytochemistry-Immunofluorescence-NBP2-52877-img0001.jpg "Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in A431 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.")
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in HeLa cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunocytochemistry-Immunofluorescence-NBP2-52877-img0002.jpg "Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in HeLa cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.")
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in MCF7 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunocytochemistry-Immunofluorescence-NBP2-52877-img0003.jpg "Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in MCF7 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.")
![Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in U251 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunocytochemistry-Immunofluorescence-NBP2-52877-img0004.jpg "Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in U251 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human kidney shows nuclear membrane positivity in renal tubules.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunohistochemistry-Paraffin-NBP2-52877-img0006.jpg "Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human kidney shows nuclear membrane positivity in renal tubules.")
![Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human placenta shows strong nuclear membrane positivity in the trophoblast.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunohistochemistry-Paraffin-NBP2-52877-img0007.jpg "Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human placenta shows strong nuclear membrane positivity in the trophoblast.")
![Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human prostate shows strong nuclear membrane immunoreactivityy in glandular cells.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunohistochemistry-Paraffin-NBP2-52877-img0008.jpg "Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human prostate shows strong nuclear membrane immunoreactivityy in glandular cells.")
![Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human skin shows strong nuclear membrane positivity in the epidermis cells.](http://images.novusbio.com/fullsize/Emerin-Antibody-CL0203-Immunohistochemistry-Paraffin-NBP2-52877-img0009.jpg "Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human skin shows strong nuclear membrane positivity in the epidermis cells.")
![Bioactivity IBSP/Sialoprotein II [Unconjugated]](https://images.novusbio.com/images/4014-sp_recombinant-human-ibsp-sialoprotein-ii-protein-cf-bioactivity-4420249391..jpg)
![Western Blot Erbin Antibody [Unconjugated]](https://images.novusbio.com/images2/Erbin_AF7866_Western_Blot_12827.jpg)
![Immunocytochemistry Erbin Antibody [Unconjugated]](https://images.novusbio.com/images2/Erbin_AF7866_Immunocytochemistry_12856.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")