![Western Blot: Emerin Antibody [NBP2-16326] - Wild-type (WT) and Emerin knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Emerin antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Emerin-Antibody-Western-Blot-NBP2-16326-img0004.jpg "Western Blot: Emerin Antibody [NBP2-16326] - Wild-type (WT) and Emerin knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Emerin antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Emerin. The exact sequence is proprietary. |
| Localization | Nucleus inner membrane; Single-pass membrane protein; Nucleoplasmic side |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EMD |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 29 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
![Immunohistochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunohistochemistry_20892.jpg)
![Immunocytochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunocytochemistry__Immunofluorescence_21143.jpg)
![Western Blot EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Western_Blot_19925.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Emerin Antibody (NBP2-16326)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Emerin Antibody [NBP2-16326] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Emerin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16326-img0003.jpg "Immunocytochemistry/Immunofluorescence: Emerin Antibody [NBP2-16326] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Emerin Antibody [NBP2-16326] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/Emerin-Antibody-Immunohistochemistry-Paraffin-NBP2-16326-img0002.jpg "Immunohistochemistry-Paraffin: Emerin Antibody [NBP2-16326] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:1000 dilution.")
![Western Blot: Emerin Antibody [NBP2-16326] - Sample (30 ug of whole cell lysate) A: HeLa nucleus 12% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/Emerin-Antibody-Western-Blot-NBP2-16326-img0001.jpg "Western Blot: Emerin Antibody [NBP2-16326] - Sample (30 ug of whole cell lysate) A: HeLa nucleus 12% SDS PAGE gel, diluted at 1:5000.")
![Bioactivity IBSP/Sialoprotein II [Unconjugated]](https://images.novusbio.com/images/4014-sp_recombinant-human-ibsp-sialoprotein-ii-protein-cf-bioactivity-4420249391..jpg)
![Western Blot Erbin Antibody [Unconjugated]](https://images.novusbio.com/images2/Erbin_AF7866_Western_Blot_12827.jpg)
![Immunocytochemistry Erbin Antibody [Unconjugated]](https://images.novusbio.com/images2/Erbin_AF7866_Immunocytochemistry_12856.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")