c-Myc Antibody (9E11) - BSA Free

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is a multifunctional, nuclear phosphoprotein that functions as a transcription factor with a theoretical molecular weight of 62kDa. However, c-Myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that a molecular weight of ~40 kDa has been observed. c-Myc is part of a heterodimeric complex with Max that acts as a potent transcriptional activator. c-Myc is modified by glycosylation and phosphorylation and has been shown to interact with numerous proteins including SMAD2, SMAD3, LSD1/KDM1A, MAD, and Sp1 (1).

A basic Helix-Loop-Helix, Leucine Zipper domain (bHLH/LZ), designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with other bHLH-Zip proteins, Mad and Mxi1. c-Myc plays a role in cell cycle progression, apoptosis, cellular transformation and angiogenesis (2). Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of cancers including B-cell Lymphomas, acute myeloid leukemia, glioblastoma, stomach adenocarcinoma, and prostate adenocarcinoma (3).

References

1. Wilkinson, D. S., Tsai, W. W., Schumacher, M. A., & Barton, M. C. (2008). Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression. Mol Cell Biol, 28(6), 1988-1998. doi:10.1128/mcb.01442-07

2. Pedrosa, A. R., Bodrug, N., Gomez-Escudero, J., Carter, E. P., Reynolds, L. E., Georgiou, P. N., . . . Hodivala-Dilke, K. M. (2019). Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861. Cancer Res, 79(17), 4371-4386. doi:10.1158/0008-5472.Can-18-3934

3. Nagasaka, M., Tsuzuki, K., Ozeki, Y., Tokugawa, M., Ohoka, N., Inoue, Y., & Hayashi, H. (2019). Lysine-Specific Demethylase 1 (LSD1/KDM1A) Is a Novel Target Gene of c-Myc. Biol Pharm Bull, 42(3), 481-488. doi:10.1248/bpb.b18-00892

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Rat, Yeast.

We have publications tested in 5 applications: Chemotaxis, ICC/IF, IHC-P, IP, WB.

Showing Publications 1 - 10 of 12. Show All 12 Publications. Collapse Publications.

Publications using NB200-108	Applications	Species
Aamir K, Sethi G, Afrin MR et al. Arjunolic acid modulate pancreatic dysfunction by ameliorating pattern recognition receptor and canonical Wnt pathway activation in type 2 diabetic rats Life sciences 2023-08-15 [PMID: 37307966]
Alghamdi TA, Krentz NAJ, Smith N et al. Zmiz1 is required for mature B-cell function and mass expansion upon high fat feeding Molecular metabolism 2022-10-25 [PMID: 36307047] (WB) Details: Dilution used in WB 1:200	WB
Cho JH, Okuma A, Sofjan K et al. Engineering advanced logic and distributed computing in human CAR immune cells Nature communications 2021-02-04 [PMID: 33542232] (ICC/IF, Human)	ICC/IF	Human
Cho S, Chae JS, Shin H et al. Hormetic dose response to L-ascorbic acid as an anti-cancer drug in colorectal cancer cell lines according to SVCT-2 expression. Sci Rep. 2018-07-27 [PMID: 30054560] (WB, Human)	WB	Human
Hubbi ME, Kshitiz, Gilkes DMet al. A nontranscriptional role for HIF-1alpha as a direct inhibitor of DNA replication. Sci Signal 2013-02-12 [PMID: 23405012]
Matsumura S et al. Activation of the Kaposi's sarcoma-associated herpesvirus major latency locus by the lytic switch protein RTA (ORF50). J Virol 79:8493-505 (2005). [PMID: 15956592] (WB, Human)	WB	Human
Sers JA. Gruppuso PA Nucleolar localization of hepatic c-Myc: a potential mechanism for c-Myc regulation. Biochim Biophys Acta 1743:141-50. 2005-01-01 [PMID: 15777849] (WB, Rat)	WB	Rat
Robert F et al. Global position recruitment of HATs HDACs in the yeast genome. Mol Cell 16:199-209. 2004-01-01 [PMID: 15494307]
Zhang H et al. The Yaf9 component of the SWR1 NuA4 complexes is required for proper gene expression, histone H4 acetylation, Htz1 replacement near telomeres. Mol Cell Biol 24:9424-36. 2004-01-01 [PMID: 15485911] (Chemotaxis, Yeast)	Chemotaxis	Yeast
Tanaka S, Diffley JF. Interdependent nuclear accumulation of budding yeast Cdt1 and Mcm2-7 during G1 phase. Nat Cell Biol4 (3):198-207. 2002-03-01 [PMID: 11836525] (IP, Yeast)	IP	Yeast
Pich A, Margaria E, Chiusa L. Oncogenes and male breast carcinoma: c-erbB-2 and p53 coexpression predicts a poor survival. J Clin Oncol 18(16):2948-56. 2000-08-01 [PMID: 10944127] (IHC-P, Human)	IHC-P	Human
Munchow S, Sauter C, Jansen RP. Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteins. J Cell Sci 112 ( Pt 10):1511-8. 1999-05-01 [PMID: 10212145] (IP, Yeast)	IP	Yeast
Show All 12 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	4 reviewed by: Western Forte	ChIP	Human	06/27/2012	View Summary Application Chromatin Immunoprecipitation Sample Tested Hela whole nuclear lysate Species Human
	5 reviewed by: Western Forte	WB	Human	06/20/2012	View Summary Application Western Blot Sample Tested HEK293 whole cell lysate Species Human
	5 reviewed by: Verified Customer	WB	Human	08/10/2010	View Summary Application Western Blot Sample Tested 293T Cells overexpressing myc-tagged proteins, Sample Amount: 50 Species Human

Array NB200-108

• c-Myc Antibodies
• c-Myc ELISA Kits
• c-Myc Lysate
• c-Myc Proteins