Conjugated antibody

Flow cytometry was developed to label and examine single cells with high throughput capacity using antibodies conjugated to fluorophores. The basic concept of flow cytometry is that a cell suspension is delivered as a single stream and is passed through a light source that uses detectors to generate data sets based off cellular properties. More specifically, the light emitted by fluorescently conjugated antibodies is channeled through selected filters to sort based off preset parameters or targets used.

Platelet Endothelial Cell Adhesion Molecule 1 (PECAM1) is a single-pass membrane receptor also known as CD31 (cluster of differentiation 31.) CD31 is expressed at high levels at endothelial cell junctions, and therefore the CD31 antibody is widely used as an endothelial marker, one use being to measure the progression of angiogenesis following recurrence of tumors.

Novus Biologicals offers the widest selection of conjugated CD4 antibody products, with a database that's continually expanding to take into account the latest developments. This includes CD4 antibodies conjugated to Dynomics and PE fluorescent dyes, for FACS analysis.

Antibody conjugation involves tagging on a protein, compound or dye, in order to track its interaction with specific antigens. It is a routine procedure in immunoassays. We at Novus Biologicals have a vast number of products in our conjugated antibody catalog, to which we are constantly adding.

A conjugated antibody (also known as a tagged, loaded or labeled antibody) is one that has been attached to a substrate such as an enzyme, toxin or inorganic compound. Modern immunoassay techniques frequently use proteins conjugated with a fluorescent dye. Contemporary antibody sources sometimes supply a wide range of both polyclonal and monoclonal conjugated antibodies.