FACS

Hematopoiesis is a complicated process that is controlled by both intrinsic and extrinsic cellular factors. It is a process of progression by which the diverse cell pedigrees that develop the blood and immune system are spawned from a shared pluripotent hematopoietic stem cell (HSC). Throughout the lifespan of an adult, two distinct hematopoietic systems are present, both resulting through embryonic development but only one of them enduring into adulthood.

Florescence activated cell sorting or Flow cytometry permits concurrent measurements of numerous florescence and light scattered events by illuming single cells or molecules in suspension as they flow through a sensing area. Distinct cells or particles could be tangibly separated corresponding to their biochemical properties and biological parameters, while the light is scattered on the molecules either in the form of forward or side scatter.

FACS (Fluorescence Activated Cell Sorting) was developed by Bonner, Sweet, Hulett, Herzenberg, and others to perform flow cytometry. Flow cytometry is a powerful analytical tool useful for the characterization or phenotypic identification of different populations of cells.

As a top worldwide antibody supplier, we at Novus Biologicals are constantly extending our antibody database to take advantage of the latest technology and product developments. Recently, we added several embryonic stem cell marker antibodies, conjugated for use in FACS (fluorescence activated cell sorting) assays.

Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and GPR49/LGR5 antibodies.

A large number of antibody suppliers supply conjugated and non-conjugated marker antibodies, targeted at specific stem cell populations. Until recently the number of oval stem cell markers was extremely limited. However, we at Novus Biologicals have succeeded in developing a new line of monoclonal antibodies specifically targeted to the murine oval cell response. These have just been added to our stem cell antibody database.

Antibody conjugation involves tagging on a protein, compound or dye, in order to track its interaction with specific antigens. It is a routine procedure in immunoassays. We at Novus Biologicals have a vast number of products in our conjugated antibody catalog, to which we are constantly adding.

A conjugated antibody (also known as a tagged, loaded or labeled antibody) is one that has been attached to a substrate such as an enzyme, toxin or inorganic compound. Modern immunoassay techniques frequently use proteins conjugated with a fluorescent dye. Contemporary antibody sources sometimes supply a wide range of both polyclonal and monoclonal conjugated antibodies.