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Desmin Antibody

 (16 Publications)
 (2 Reviews)
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Summary
Product Discontinued
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    • Catalog Number
      NB120-15200
    • Availability
      Product Discontinued

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Desmin Antibody Summary

Immunogen
Synthetic peptide mapping near the C-terminus of human desmin.
Localization
Cytoplasmic
Specificity
Desmin
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DES
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:75-1:200
  • Western Blot
Application Notes
IF use reported in literature (PMID 22427904). Immunohistochemistry-Frozen was reported in scientific literature. Use in Western blot reported in scientific literature (PMID 24481605)
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Desmin Overexpression Lysate
Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Human Leiomysarcoma Whole Tissue Lysate (Adult Whole Tumor)
Reviewed Applications
Read 2 Reviews rated 5
using
NB120-15200 in the following applications:
Publications
Read Publications using
NB120-15200 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence homology: Bovine, Chicken, Dog, Frog, Pig and Rat, Canine, Porcine, Rabbit. Sheep reactivity reported in scientific literature (PMID: 25639519)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.6) and 1.0% BSA
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Desmin Antibody

  • CMD1I
  • CMD1IFLJ41013
  • CSM1
  • CSM2
  • DES
  • Desmin
  • FLJ12025
  • FLJ39719
  • FLJ41793
  • intermediate filament protein

Background

Desmin is an intermediate filament protein of both smooth and striated muscles. Antibody to desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z bands giving a characteristic striated appearance. Antidesmin antibody is useful in identification of tumors of myogenic origin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Desmin Antibody (NB120-15200)(16)

We have publications tested in 3 confirmed species: Human, Mouse, Sheep.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(8)
IHC
(2)
IHC-Fr
(2)
IHC-P
(2)
WB
(3)
All Applications
Filter By Species
Human
(4)
Mouse
(8)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB120-15200 Applications Species
Iguchi N, Carrasco A, Xie AX, et al. Functional constipation induces bladder overactivity associated with upregulations of Htr2 and Trpv2 pathways Scientific reports Jan 13 2021 [PMID: 33441874] (ICC/IF, Mouse) ICC/IF Mouse
Kikuchi A, Singh S, Poddar M et al. HEPATIC STELLATE CELL-SPECIFIC PLATELET-DERIVED GROWTH FACTOR RECEPTOR ALPHA LOSS REDUCES FIBROSIS AND PROMOTES REPAIR FOLLOWING HEPATOCELLULAR INJURY Am. J. Pathol. Jun 29 2020 [PMID: 32615075] (IHC) IHC
Barisic D, Erb M, Follo M et al. Lack of a skeletal muscle phenotype in adult human bone marrow stromal cells following xenogeneic-free expansion Stem Cell Res Ther Feb 22 2020 [PMID: 32087752] (ICC/IF, Human) ICC/IF Human
Almonacid Suarez AM, Zhou Q, van Rijn P, Harmsen MC Directional topography gradients drive optimum alignment and differentiation of human myoblasts J Tissue Eng Regen Med Nov 1 2019 [PMID: 31677226] (ICC/IF, Human) ICC/IF Human
Iguchi N, Donmez MI, Carrasco A et al. Doxorubicin induces detrusor smooth muscle impairments through myosin dysregulation, leading to a risk of lower urinary tract dysfunction Am. J. Physiol. Renal Physiol. May 8 2019 [PMID: 31066574] (WB, Mouse) WB Mouse
Singh BN, koyano-Nakagawa N, Gong W et al. A conserved HH-Gli1-Mycn network regulates heart regeneration from newt to human. Nat Commun. Oct 12 2018 [PMID: 30315164] (IHC-Fr, Mouse) IHC-Fr Mouse
Hwang JY, Mannowetz N, Zhang Y et al. Elimination of Wnt Secretion From Stellate Cells Is Dispensable for Zonation and Development of Liver Fibrosis Following Hepatobiliary Injury Gene Expr. Apr 18 2019 [PMID: 30236172] (IHC-P, ICC/IF, WB, Mouse) IHC-P, ICC/IF, WB Mouse
Chen Y, Wu Z, Yuan B et al. MicroRNA-146a-5p attenuates irradiation-induced and LPS-induced hepatic stellate cell activation and hepatocyte apoptosis through inhibition of TLR4 pathway. Cell Death Dis. Jan 18 2018 [PMID: 29348414] (ICC/IF, Human) ICC/IF Human
Barnabei MS, Sjaastad FV, Townsend D et al. Severe dystrophic cardiomyopathy caused by the enteroviral protease 2A-mediated C-terminal dystrophin cleavage fragment Sci Transl Med. 2015 Jul 01 [PMID: 26136477]
Castillo-Melendez M, Yawno T, Allison BJ et al. Cerebrovascular adaptations to chronic hypoxia in the growth restricted lamb Int. J. Dev. Neurosci. 2015 Jan 29 [PMID: 25639519] (IHC-P, Sheep) IHC-P Sheep
Show All 16 Publications.

Reviews for Desmin Antibody (NB120-15200) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB120-15200:
Filter by Applications
IHC-Fr
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Desmin NB120-15200
Enlarge 		5
reviewed by:
Margherita verardo
 IHC-Fr Human 06/12/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedHuman liver
SpeciesHuman
Lot160407LVC
Immunohistochemistry-Paraffin Desmin NB120-15200
Enlarge 		5
reviewed by:
Verified Customer
 IHC-P Mouse 05/02/2012
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedE11 mouse embryos
SpeciesMouse
CommentsThis is a great antibody. The example given is a fixed paraffin embedded slide with Dako antigen retrieval. However, it has also worked without AR, with other AR methods, and on cryosections. It has worked well in the heart for every method that I have attempted for E8-E11 mouse embryos.

Blocking

Blocking DetailsBlocked with IHC buffer for 1 hour at RT (IHC buffer: 2% normal donkey serum, 1% bovine serum albumin, 0.3% Triton X-100, 0.02%

Primary Anitbody

Dilution Ratio1:200 in IHC buffer, overnight at 4C

Secondary Antibody

Secondary DescriptionJackson ImmunoResearch donkey anti rabbit Cy3, 1:200, and Jackson ImmunoResearch donkey anti-Chicken-dylight 488,

Details

Detection NotesResults were imaged on a Zeiss Axio Imager M1 upright microscope
Fixation Details4% PFA, Antigen retrieval with Dako AR buffer in 95C water bath for 20 minutes, cooled at RT for 30 minutes
Wash DescriptionWashed with PBS 3 times for 5 minutes each

Comments

CommentsThis is a great antibody. The example given is a fixed paraffin embedded slide with Dako antigen retrieval. However, it has also worked without AR, with other AR methods, and on cryosections. It has worked well in the heart for every method that I have attempted for E8-E11 mouse embryos.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Desmin Antibody (NB120-15200) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Desmin Overexpression Lysate
Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Human Leiomysarcoma Whole Tissue Lysate (Adult Whole Tumor)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0
Immunohistochemistry-Frozen Desmin NB120-15200
Enlarge
 Margherita verardo
06/12/2018
Application: IHC-Fr
Species: Human
read this review
Immunohistochemistry-Paraffin Desmin NB120-15200
Enlarge
 Verified Customer
05/02/2012
Application: IHC-P
Species: Mouse
read this review

Bioinformatics

Gene Symbol DES
Entrez
Uniprot