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Connexin 43/GJA1 [p Ser367] Antibody

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Summary
Product Discontinued
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    • Catalog Number
      NB100-81868
    • Availability
      Product Discontinued

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Connexin 43/GJA1 [p Ser367] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Connexin43 around the phosphorylation site of serine 367 (R-A-S-S-R).
Modification
p Ser367
Marker
Gap Junctions Marker
Specificity
Connexin43 (specific to Phospho-Ser367) detects endogenous levels of Connexin43 only when phosphorylated at serine 367.
Clonality
Polyclonal
Host
Rabbit
Gene
GJA1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:20000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Adrenal Membrane Tissue Lysate (Adult Membrane Normal)
Human Adrenal Whole Tissue Lysate (Adult Whole Normal)
Connexin 43/GJA1 Overexpression Lysate
A-431 Membrane Cell Lysate

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Connexin 43/GJA1 [p Ser367] Antibody

  • Connexin 43
  • connexin-43
  • CX43
  • CX43gap junction protein, alpha-like
  • DFNB38
  • Gap junction 43 kDa heart protein
  • gap junction alpha-1 protein
  • gap junction protein, alpha 1, 43kDa (connexin 43)
  • gap junction protein, alpha 1, 43kDa
  • GJA1
  • GJAL
  • HSS
  • ODD
  • ODDD
  • ODOD
  • SDTY3

Background

Connexin 43 aka GJA1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. GJA1 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. GJA1 is targeted by several protein kinases that regulate myocardial cell cell coupling. A related intronless GJA1 pseudogene, GJA1P, has been mapped to chromosome 5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Connexin 43/GJA1
[p Ser367] Antibody
NB100-81868
Connexin 43/GJA1 [p Ser367] Antibody
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC

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Control Lysate(s)

Human Adrenal Membrane Tissue Lysate (Adult Membrane Normal)
Human Adrenal Whole Tissue Lysate (Adult Whole Normal)
Connexin 43/GJA1 Overexpression Lysate
A-431 Membrane Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Research Areas for Connexin 43/GJA1 Antibody (NB100-81868)

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Blogs on Connexin 43/GJA1.

Check out the latest blog posts on Connexin 43/GJA1.
Connexin: Bridging the Gap of Intercellular Communication
Connexin 43/GJA1 is a member of the connexin gene family and the most abundant protein component found within gap junctions. Gap junctions are the cell-to-cell contacts that provide direct intercellular communication between cells by regulating back a...  Read full blog post.
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Bioinformatics

Gene Symbol GJA1
Entrez
Uniprot