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Related Links Flyer: Antibodies for COVID-19 Research |
SARS-CoV-2 Research ResourcesAfter SARS-CoV-2 enters the host cell the viral genomic RNA is translated from two open reading frames (ORFs), ORF1a and ORF1b. The encoded polypeptides, pp1a and pp1ab, are further processed to produce 11 and 16 non-structural proteins (nsps), respectively. These nsps are required for viral replication and pathogenesis. In addition to primary translation, subgenomic RNAs (sgRNAs) are generated by discontinuous transcription and deletions, allowing for translation of both the structural and ORF accessory proteins. The 9 ORF accessory proteins appear to have diverse roles involving host-virus interactions and viral pathogenesis. While the main structural proteins (Spike, Nucleocapsid, Envelope and Membrane) have been well characterized, the ORF accessory proteins and nsps are in general less understood. View Products by SARS-CoV-2 Target
What are SARS-CoV-2 Non-structural Proteins?Non-structural proteins (nsps) serve different functions in the viral replication cycle. Together the encoded nsps (1-16) form a replicase-transcriptase complex.
Antibodies to SARS-CoV Non-structural ProteinsNovus offers rabbit polyclonal antibodies to nsp5, nsp8, and nsp13 proteins which are validated in several applications (e.g., WB, ICC/IF, ELISA, IP and IM). Novus Innovator's Reward Program allows you to test some of these available antibodies for the detection of SARS-CoV-2. Additionally, through Novus' 100% guarantee you can test antibodies against SARS-CoV-2 targets sharing 90% or greater homology with proteins in SARS-CoV. Learn more about our 100% guarantee and Innovator's Reward Program.
SARS-CoV-2 Recombinant Proteins
Antibodies to SARS-CoV-2 Open Reading Frames (ORF) Accessory ProteinsNovus offers multiple rabbit polyclonal antibodies to the Open Reading Frame (ORF) Accessory Proteins of SARS-CoV-2 including ORF3a, ORF6, OFR7a, ORF8, and ORF10. Although the sequence identity for most accessory protein ORFs is greater than 85% between SARS-CoV-2 and SARS-CoV, ORF3, ORF8, and ORF10 show less similarity. The functionality of the SARS-CoV-2 ORF accessory proteins varies widely, though they all contribute to viral pathogenesis. These antibodies have been validated for use in ELISA and WB. Additionally, through Novus' 100% guarantee you can test antibodies for applications that aren't yet validated. Learn more about our 100% guarantee and Innovator's Reward Program.
Select ReferencesAhmadpour, D., & Ahmadpoor, P. (2020). How the COVID-19 Overcomes the Battle? An Approach to Virus Structure. Iranian journal of kidney diseases. Angelini, M. M., Akhlaghpour, M., Neuman, B. W., & Buchmeier, M. J. (2013). Severe acute respiratory syndrome coronavirus nonstructural proteins 3, 4, and 6 induce double-membrane vesicles. MBio. https://doi.org/10.1128/mBio.00524-13 Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Hüttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M., … Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9 Kim, D., Lee, J.-Y., Yang, J.-S., Kim, J. W., Kim, V. N., & Chang, H. (2020). The architecture of SARS-CoV-2 transcriptome. Cell. https://doi.org/10.1016/j.cell.2020.04.011 Mohammad, S., Bouchama, A., Mohammad Alharbi, B., Rashid, M., Saleem Khatlani, T., Gaber, N. S., & Malik, S. S. (2020). SARS-CoV-2 ORF8 and SARS-CoV ORF8ab: Genomic Divergence and Functional Convergence. Pathogens. https://doi.org/10.3390/pathogens9090677 Prajapat, M., Sarma, P., Shekhar, N., Avti, P., Sinha, S., Kaur, H., … Medhi, B. (2020). Drug targets for corona virus: A systematic review . Indian Journal of Pharmacology. https://doi.org/10.4103/ijp.IJP_115_20 Ren, Y., Shu, T., Wu, D., Mu, J., Wang, C., Huang, M., Han, Y., Zhang, X. Y., Zhou, W., Qiu, Y., & Zhou, X. (2020). The ORF3a protein of SARS-CoV-2 induces apoptosis in cells. Cellular & molecular immunology. https://doi.org/10.1038/s41423-020-0485-9 Wu, C., Liu, Y., Yang, Y., Zhang, P., Zhong, W., Wang, Y., … Li, H. (2020). Analysis of therapeutic targets for SARS-CoV-2 and discovery of potential drugs by computational methods . Acta Pharmaceutica Sinica B. https://doi.org/10.1016/j.apsb.2020.02.008 Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The protein journal. https://doi.org/10.1007/s10930-020-09901-4 |
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