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SARS-CoV-2 ORF10 Antibody - BSA Free

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Product Details

Summary
Reactivity VSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

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SARS-CoV-2 ORF10 Antibody - BSA Free Summary

Immunogen
Synthetic peptide
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ORF10
Purity
Epitope affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:500-1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 10% glycerol, pH7.2
Preservative
0.02% Sodium Azide
Purity
Epitope affinity purified

Alternate Names for SARS-CoV-2 ORF10 Antibody - BSA Free

  • 2019-nCoV ORF10 Protein
  • 2019-nCoV ORF10
  • COVID-19 ORF10
  • Human coronavirus ORF10 Protein
  • ORF10 protein
  • ORF10
  • SARSCoV2 ORF10 Protein
  • SARS-CoV-2 ORF10 Protein
  • SARS-CoV-2
  • Severe Acute Respiratory Syndrome Coronavirus 2 ORF10 Protein

Background

SARS-CoV-2 Open Reading Frame 10 (ORF10) is one of the nine downstream accessory protein open reading frames of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 ORF10 is the smallest accessory protein at 38 amino acids (aa) in length with a theoretical molecular weight of 4.4 kDa (1, 2). ORF10 is proposed to be unique to SARS-CoV-2, however it likely evolved from mutations in other coronaviruses (1, 3). Interestingly, the ORF10 of SARS-CoV-2 has 97.3% homology to Pangolin-CoV ORF10, but there are still prominent differences in properties such as protein structure, solubility, and mutational diversification (4).

Functionally, SARS-CoV-2 ORF10 is suggested to interact with the Cullin 2 RING E3 ligase complex, indicating that ORF10 might promote viral pathogenesis by taking over the ubiquitination machinery (4, 5). Additionally, the SARS-CoV-2 ORF10 gene appears to be differentially expressed based on COVID-19 severity. For example, ORF10 appears to be highly expressed in the infected cells from severe cases while it is either lowly expressed or not detectable in cells infected from moderate cases (6). Therefore, ORF10 expression could be indicative of COVID-19 progression (6).

References

1. Michel, C. J., Mayenr, C., Poch, O., & Thompson, J. D. (2020). Characterization of accessory genes in coronavirus genomes. Virology journal. https://doi.org/10.1186/s12985-020-01402-1

2. Uniprot (A0A6B9W2G5)

3. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The protein journal. https://doi.org/10.1007/s10930-020-09901-4

4. Hassan, S., Attrish, D., Ghosh, S., Choudhury, P., Uversky, V., Uhal, B., Lundstrom, K., Rezaei, N., Aljabali, A., Seyran, M., et al. (2020). Notable sequence homology of the ORF10 protein introspects the architecture of SARS-COV-2. bioRxiv. 10.1101/2020.09.06.284976.

5. Ahmadpour, D., & Ahmadpoor, P. (2020). How the COVID-19 Overcomes the Battle? An Approach to Virus Structure. Iranian journal of kidney diseases.

6. Liu, T., Jia, P., Fang, B., & Zhao, Z. (2020). Differential Expression of Viral Transcripts From Single-Cell RNA Sequencing of Moderate and Severe COVID-19 Patients and Its Implications for Case Severity. Frontiers in microbiology. https://doi.org/10.3389/fmicb.2020.603509

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SARS-CoV-2 ORF10 Antibody (NBP3-05710) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional SARS-CoV-2 ORF10 Products

Array NBP3-05710

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Bioinformatics

Gene Symbol ORF10