0.5 μg and 1 μg of Recombinant SARS-CoV-2 Papain-like Protease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
Recombinant SARS-CoV-2 virus Papain-like protease (PLPro) is a Ubiquitin- and ISG15-deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial PLPro concentration of 20-100 nM when using Ubiquitin-Rhodamine 110 (U-555) substrate. When using polyubiquitin chain substrate(s), this enzyme demonstrates a preference for K48 linkages.
Source
E. coli-derived sars-cov-2 Papain-like Protease protein Glu746 - Lys1060, with an N-terminal GST-tag
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Applications/Dilutions
Dilutions
Bioactivity
Theoretical MW
62 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using E-611 in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 °C as supplied.
3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in HEPES, NaCl, Glycerol and TCEP.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF
Papain-like Protease
PLpro
PL-PRO
pp1a
Replicase polyprotein 1a
Background
The Papain-like protease (PLPro) from the human SARS-CoV-2 coronavirus (Severe Acute Respiratory Syndrome coronavirus 2 is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. In other coronaviruses, PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminal end of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISG15ylating activity. In vivo, this protease antagonizes innate immunity by acting on IFN beta and NF- kappa B signaling pathways. When used in vitro with polyubiquitin substrates, the enzyme demonstrates a strong preference for K48 linkages.
Clasman J.R., et al. (2020) Antiviral Res. 174: 104661.
Frieman M., et al. (2009) J. Virol. 83: 6689.
Lindner H.A., et al. (2007) Arch. Biochem. Biophys. 466: 8.
Ratia K., et al. (2014) PLoS Pathog. doi: 10.1371/journal.ppat.1004113.
Publications for Papain-like Protease (E-611)(2)
We have publications tested in 2 confirmed species: Mouse, N/A.
We have publications tested in 2 applications: In Vivo, Inhibition.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
=
÷
Review this Product
Be the first to review our Recombinant SARS-CoV-2 GST-Papain-like Protease, CF and receive a gift card or discount.
