![Immunocytochemistry/Immunofluorescence: SARS-CoV nsp13 Antibody [NBP2-89168] - Immunofluorescence microscopy using anti-SARS-CoV nsp13 antibody, 6-h post infection Vero-E6 cells. For detection Cy3 conjugated Goat-anti-Rabbit IgG MX (NB120-6939) was used.](http://images.novusbio.com/fullsize/SARS-CoV-nsp3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-89168-img0002.jpg "Immunocytochemistry/Immunofluorescence: SARS-CoV nsp13 Antibody [NBP2-89168] - Immunofluorescence microscopy using anti-SARS-CoV nsp13 antibody, 6-h post infection Vero-E6 cells. For detection Cy3 conjugated Goat-anti-Rabbit IgG MX (NB120-6939) was used.")
| Immunogen | The antibody was developed by immunizing rabbits with a BSA-coupled synthetic peptide corresponding to amino acids 584-604 (C-klqftsleiprrnvatlq) from the N (Nonstructural protein 13) for the Human SARS coronavirus. |
| Specificity | This antibody is directed against SARS-Coronavirus nsp13 protein. |
| Predicted Species | SARS-CoV-2 (100%). Backed by our 100% Guarantee. |
| Isotype | Serum |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | orf1ab |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | This antibody has been tested for use in western blotting, immunofluorescence microscopy and immunoelectron microscopy. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 67 kDa in size corresponding to SARS-CoV nsp13 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37C. Infection occurred in PBS/DEAE/2% FCS followed by exchange to EMEM/25mMHEPES/2% FCS. Cells were fixed with PBS/3% PFA. After washing fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min. |
|
| Theoretical MW | 66.87 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Publications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | Antiserum |
| Preservative | 0.01% Sodium Azide |
| Purity | Whole Antiserum |
| Publication using NBP2-89168 | Applications | Species |
|---|---|---|
| Meng W, Guo S, Cao S et al. Development and characterization of a new monoclonal antibody against SARS-CoV-2 NSP12 (RdRp) Journal of medical virology 2022-10-21 [PMID: 36271490] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | orf1ab |
