Recombinant SARS-CoV-2 3CL Protease (Avi Epitope Tag) His Avi-tag Protein Summary
| Description |
Recombinant Virus SARS-CoV-2 3CL Protease is produced by E. coli expression system. The target protein is expressed with sequence (Ser1-Gln306) of 2019-ncov 3C-like Proteinase (Accession #YP_009725301.1) fused with a 6×His,Avi tag at the N-terminus. |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
ORF1ab |
| Purity |
>95%, by SDS-PAGE |
| Endotoxin Note |
< 1.0 EU/ug of the protein by LAL method. |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer |
Supplied as a 0.22 um filtered solution in 20mM HEPES, 120mM NaCl, 10% Glycerol, 2mM TCEP, pH7.4 |
| Preservative |
No Preservative |
| Purity |
>95%, by SDS-PAGE |
Alternate Names for Recombinant SARS-CoV-2 3CL Protease (Avi Epitope Tag) His Avi-tag Protein
Background
Generally, viruses have proteases to process their proteins into active form. Because of its pivotal role in the viral life cycle, proteases are primary targets for the development of antiviral agents. 3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase. 3CL protease is the only Coronavirus protein for which structural information is available. 3CL protease comprises three domains, the substrate-binding site is expected to be located between domains I and II, and domain III is a globular cluster comprising five helices. 3CL protease is a homodimer.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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![SDS-Page: Recombinant SARS-CoV-2 3CL Protease (Avi Epitope Tag) His Avi-tag Protein [NBP2-92992] - Recombinant Virus SARS-CoV-2 3CL Protease Avi Epitope Tag Protein [NBP2-92992] - Recombinant Virus SARS-CoV-2 3CL Protease with His and Avi tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 38 kDa.](http://images.novusbio.com/fullsize/Recombinant-SARS-CoV-2-3CL-Protease-Avi-Epitope-Tag-His-Avi-tag-Protein-SDS-Page-NBP2-92992-img0001.jpg "SDS-Page: Recombinant SARS-CoV-2 3CL Protease (Avi Epitope Tag) His Avi-tag Protein [NBP2-92992] - Recombinant Virus SARS-CoV-2 3CL Protease Avi Epitope Tag Protein [NBP2-92992] - Recombinant Virus SARS-CoV-2 3CL Protease with His and Avi tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 38 kDa.")
