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Yes Antibody

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Western Blot: Yes Antibody [NBP1-85369] - Analysis in human cell line U-2 OS.
Immunocytochemistry/ Immunofluorescence: Yes Antibody [NBP1-85369] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: Yes Antibody [NBP1-85369] - Staining of human kidney shows moderate cytoplasmic positivity in tubule and glomerular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Yes Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KSKENKSPAIKYRPENTPEPVSTSVSHYGAEPTTVSPCPSSSAKGTAVNFSSLS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YES1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Yes Protein (NBP1-85369PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Yes Antibody

  • cellular yes-1 protein
  • c-yes
  • EC 2.7.10
  • EC 2.7.10.2
  • HsT441
  • P61-YES
  • Proto-oncogene c-Yes
  • proto-oncogene tyrosine-protein kinase Yes
  • v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
  • Yamaguchi sarcoma oncogene
  • Yes
  • YES1

Background

Yes1 is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol YES1