![Immunohistochemistry-Paraffin: YB1 Antibody (YBX1/2430) [NBP3-08774] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with YB1 Mouse Monoclonal Antibody (YB1/2430)](http://images.novusbio.com/fullsize/YB1-Antibody-YBX1-2430-Immunohistochemistry-Paraffin-NBP3-08774-img0001.jpg "Immunohistochemistry-Paraffin: YB1 Antibody (YBX1/2430) [NBP3-08774] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with YB1 Mouse Monoclonal Antibody (YB1/2430)")
| Reactivity | HuSpecies Glossary |
| Applications | IHC, MA |
| Clone | YBX1/2430 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07452). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human full-length YB1 protein (Uniprot: P67809) |
| Localization | Cytoplasmic and Nuclear |
| Marker | Tumor Biomarker |
| Specificity | The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Y-box binding protein-1 (YBX1) is the prototypic member of the cold shock protein family that fulfills numerous cellular functions. In the nucleus, YBX1 protein orchestrates transcription of proliferation-related genes, whereas in the cytoplasm it associates with mRNA and directs translation. In human tumor entities, such as breast, lung and prostate cancer, cellular YBX1 expression indicates poor clinical outcome, suggesting that YBX1 is an attractive marker to predict patients' prognosis. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | YBX1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. |
| Theoretical MW | 36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for YB1 Antibody (NBP3-08774)Find related products by research area.
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Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff... Read full blog post. |
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New Techniques Using Phosphoserine Antibodies Phosphoserine, the phosphorylated modification of the amino acid serine, is a central post-translational modification within a cell for many biological and biomedical processes. The phosphorylation of specifically four residue types - histidine, serin... Read full blog post. |
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| Gene Symbol | YBX1 |
![Protein Array: YB1 Antibody (YBX1/2430) [NBP3-08774] - Analysis of Protein Array containing more than 19,000 full-length human proteins using YB1 Mouse Monoclonal Antibody (YB1/2430).](http://images.novusbio.com/fullsize/YB1-Antibody-YBX1-2430-Protein-Array-NBP3-08774-img0002.jpg "Protein Array: YB1 Antibody (YBX1/2430) [NBP3-08774] - Analysis of Protein Array containing more than 19,000 full-length human proteins using YB1 Mouse Monoclonal Antibody (YB1/2430).")
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