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YB1 Antibody (4F12)

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Immunocytochemistry/ Immunofluorescence: YB1 Antibody (4F12) [H00004904-M01] - Analysis of monoclonal antibody to YBX1 on HeLa cell. Antibody concentration 20 ug/ml.
ELISA: YB1 Antibody (4F12) [H00004904-M01] - Detection limit for recombinant GST tagged YBX1 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
4F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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YB1 Antibody (4F12) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
YBX1 (NP_004550, 51 a.a. ~ 139 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DKKVIATKVLGTVKWFNVRNGYGFINRNDTKEDVFVHQTAIKKNNPRKYLRSVGDGETVEFDVVEGEKGAEAANVTGPGGVPVQGSKYA
Specificity
YBX1 - nuclease sensitive element binding protein 1
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
YBX1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IF and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for YB1 Antibody (4F12)

  • BP-8
  • CBF-A
  • class II, Y box-binding protein I
  • CSDA2
  • CSDB
  • DBPB CCAAT-binding transcription factor I subunit A
  • EFI-A
  • Enhancer factor I subunit A
  • MDR-NF1
  • MGC104858
  • MGC110976
  • MGC117250
  • NSEP1 Y-box-binding protein 1
  • nuclease-sensitive element-binding protein 1
  • Y box binding protein 1
  • YB1 DNA-binding protein B
  • YB-1 Y-box transcription factor
  • YBX1

Background

The Y-box binding protein 1 (YB1) is a pluripotent DNA/RNA-binding factor which regulates gene expression through transcription and translation. YB1 has been shown to be a marker of tumor aggressiveness and belongs to the cold-shock domain family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for YB1 Antibody (H00004904-M01) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for YB1 Antibody (H00004904-M01)

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Blogs on YB1.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff...  Read full blog post.

New Techniques Using Phosphoserine Antibodies
Phosphoserine, the phosphorylated modification of the amino acid serine, is a central post-translational modification within a cell for many biological and biomedical processes. The phosphorylation of specifically four residue types - histidine, serin...  Read full blog post.

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Bioinformatics

Gene Symbol YBX1
Entrez
OMIM
Uniprot