![Western Blot: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541] - Whole cell lysate (50 ug) from asynchronously growing HeLa (H), 293T (T) and mouse NIH-3T3 (M) cells. Antibody used at 0.1 ug/ml. The binding of the antibody was blocked by pre-incubation with the phospho-peptide but was not blocked by pre-incubation with](http://images.novusbio.com/fullsize/XRCC1-[p-Ser485--p-Thr488]-Antibody-Western-Blot-NB100-541-img0003.jpg "Western Blot: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541] - Whole cell lysate (50 ug) from asynchronously growing HeLa (H), 293T (T) and mouse NIH-3T3 (M) cells. Antibody used at 0.1 ug/ml. The binding of the antibody was blocked by pre-incubation with the phospho-peptide but was not blocked by pre-incubation with")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region of surrounding residues Ser 485 and Thr 488 of human X-ray Repair Complementing protein 1 using the numbering given in SwissProt entry P18887 (GeneID 7515). |
| Modification | p Ser485, p Thr488 |
| Specificity | NB100-541 is specific for human phospho-XRCC1 protein. |
| Predicted Species | Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | XRCC1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal working dilutions should be determined experimentally by the investigator. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| Control |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![XRCC1 [p Ser485, p Thr488] Antibody](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "XRCC1 [p Ser485, p Thr488] Antibody")
Secondary Antibodies |
Isotype Controls |
Research Areas for XRCC1 Antibody (NB100-541)Find related products by research area.
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PARP Antibody Assays aid both Apoptosis and Cancer Research The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play... Read full blog post. |
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![Immunohistochemistry-Paraffin: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541] - Sample: FFPE sections of human stomach carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- Phospho-XRCC1-S485/T488 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/XRCC1-[p-Ser485--p-Thr488]-Antibody-Immunohistochemistry-Paraffin-NB100-541-img0005.jpg "Immunohistochemistry-Paraffin: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541] - Sample: FFPE sections of human stomach carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- Phospho-XRCC1-S485/T488 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")