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XRCC1 Antibody (OTI2F8) [Janelia Fluor® 635]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI2F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 635

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74891AF350
Alexa Fluor 405
NBP2-74891AF405
Alexa Fluor 488
NBP2-74891AF488
Alexa Fluor 532
NBP2-74891AF532
Alexa Fluor 594
NBP2-74891AF594
Alexa Fluor 647
NBP2-74891AF647
Alexa Fluor 700
NBP2-74891AF700
Alexa Fluor 750
NBP2-74891AF750
Biotin
NBP2-74891B
CoraFluor 1
NBP2-74891CL1
DyLight 350
NBP2-74891UV
DyLight 405
NBP2-74891V
DyLight 488
NBP2-74891G
DyLight 550
NBP2-74891R
DyLight 594
NBP2-74891DL594
DyLight 650
NBP2-74891C
DyLight 680
NBP2-74891FR
DyLight 755
NBP2-74891IR
FITC
NBP2-74891F
HRP
NBP2-74891H
Janelia Fluor 525
NBP2-74891JF525
Janelia Fluor 549
NBP2-74891JF549
Janelia Fluor 585
NBP2-74891JF585
Janelia Fluor 646
NBP2-74891JF646
Janelia Fluor 669
NBP2-74891JF669
Unconjugated
NBP2-02280
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-02280
Azide and BSA Free
NBP2-74891
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XRCC1 Antibody (OTI2F8) [Janelia Fluor® 635] Summary

Immunogen
Full length human recombinant protein of human XRCC1 (NP_006288) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
XRCC1
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for XRCC1 Antibody (OTI2F8) [Janelia Fluor® 635]

  • DNA repair protein XRCC1
  • RCC
  • X-ray repair complementing defective repair in Chinese hamster cells 1
  • X-ray repair cross-complementing protein 1
  • X-ray-repair, complementing defective, repair in Chinese hamster

Background

XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA Ligase III-a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol XRCC1