Novus Biologicals products are now on bio-techne.com

XRCC1 Antibody (OTI2F8) - Azide and BSA Free

Images

 
Western Blot: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunocytochemistry/ Immunofluorescence: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY XRCC1.
Immunohistochemistry: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891] - Staining of paraffin-embedded pancreas tissue using anti-XRCC1 mouse monoclonal antibody.
Immunohistochemistry: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-XRCC1 mouse monoclonal antibody.
Immunohistochemistry: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-XRCC1 mouse monoclonal antibody.
Immunohistochemistry: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891] - staining of paraffin-embedded bladder tissue using anti-RC204952 mouse monoclonal antibody.
Immunohistochemistry: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-XRCC1 mouse monoclonal antibody.
Immunohistochemistry: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891] - Staining of paraffin-embedded colon tissue using anti-XRCC1 mouse monoclonal antibody.
Immunohistochemistry: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891] - Staining of paraffin-embedded Kidney tissue using anti-XRCC1 mouse monoclonal antibody.
Immunohistochemistry: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891] - Staining of paraffin-embedded lung tissue using anti-XRCC1 mouse monoclonal antibody.
Immunohistochemistry: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891] - Staining of paraffin-embedded prostate tissue using anti-XRCC1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI2F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

XRCC1 Antibody (OTI2F8) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human XRCC1 (NP_006288) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
XRCC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
69.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for XRCC1 Antibody (OTI2F8) - Azide and BSA Free

  • DNA repair protein XRCC1
  • RCC
  • X-ray repair complementing defective repair in Chinese hamster cells 1
  • X-ray repair cross-complementing protein 1
  • X-ray-repair, complementing defective, repair in Chinese hamster

Background

XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA Ligase III-a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3296
Species: Hu, Mu, Rt
Applications: WB
NB100-116
Species: Hu, Mu, Pm, Rt
Applications: ChIP, ELISA, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, PLA, Simple Western, WB
NB100-165
Species: Dr, Ha, Hu
Applications: ICC/IF (-), IP, WB
NB100-106
Species: Hu, Mu, Po, Pm, Rb, Rt
Applications: ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, WB
202-IL
Species: Hu
Applications: BA
NBP2-27335
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, WB
NB600-326
Species: ET
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
DVE00
Species: Hu
Applications: ELISA
NB600-607
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-45411
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NB500-704
Species: ChHa, Hu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-16756
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-41190
Species: Ch, Hu, Mu
Applications: B/N, ICC/IF, IP, PLA, WB
NBP2-49671
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-88370
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-32733
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP2-38600
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-417
Species: Hu, Mu, Pl, Rt
Applications: ChIP, Dual ISH-IHC, ELISA, Flow, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, MiAr, PLA, Simple Western, WB
NBP2-74891
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

Publications for XRCC1 Antibody (NBP2-74891) (0)

There are no publications for XRCC1 Antibody (NBP2-74891).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for XRCC1 Antibody (NBP2-74891) (0)

There are no reviews for XRCC1 Antibody (NBP2-74891). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for XRCC1 Antibody (NBP2-74891) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional XRCC1 Products

Research Areas for XRCC1 Antibody (NBP2-74891)

Find related products by research area.

Blogs on XRCC1.

PARP Antibody Assays aid both Apoptosis and Cancer Research
The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our XRCC1 Antibody (OTI2F8) - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol XRCC1