XIAP Antibody (117318)

Images

 
XIAP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 25 µg/mL Mouse Anti-Human XIAP Monoclonal Antibody (Catalog # MAB8221) overnight at 4 °C. Tissue was stained with the ...read more
Recombinant Human Caspase-7 (0.012 μg/mL, Catalog # 823-C7) activity is measured in the presence of Recombinant Human XIAP (2.75 μg/mL, Catalog # 822‑XF) that has been preincubated with increasing concentrations of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, B/N
Clone
117318
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

XIAP Antibody (117318) Summary

Immunogen
E. coli-derived recombinant human XIAP
Met1-Ser497
Accession # P98170
Specificity
Detects human XIAP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) cIAP-1 or rh cIAP-2 is observed. Neutralizes human XIAP and human XIAP-BIR2 bioactivity. Does not neutralize XIAP-BIR3 or Livin bioactivity.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
XIAP
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 10 ug/mL
Reviewed Applications
Read 2 Reviews rated 3
using
MAB8221 in the following applications:

Publications
Read Publication using
MAB8221 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XIAP Antibody (117318)

  • API3X-linked inhibitor of apoptosis protein
  • apoptosis inhibitor 3
  • baculoviral IAP repeat-containing 4
  • baculoviral IAP repeat-containing protein 4
  • BIRC4
  • BIRC4XLP2
  • E3 ubiquitin-protein ligase XIAP
  • EC 6.3.2.-
  • hIAP3
  • hIAP-3
  • hILP
  • IAP3
  • IAP-3
  • IAP-like protein
  • ILP
  • ILP1
  • Inhibitor of apoptosis protein 3
  • MIHA
  • XIAP
  • X-linked IAP
  • X-linked inhibitor of apoptosis

Background

XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits caspase-3 and caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for XIAP Antibody (MAB8221)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC.


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ICC
(1)
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Mouse
(1)
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Reviews for XIAP Antibody (MAB8221) (2) 32

Average Rating: 3
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using MAB8221:
Filter by Applications
IHC-P
(1)
Flow
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IHC-P Human 04/03/2022
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBrain tissue
SpeciesHuman
Lot117318

Comments

Comments25 ug/mL dilution for ihc
Flow Cytometry XIAP MAB8221
Enlarge
1
reviewed by:
Verified Customer
Flow Human 12/11/2016
View

Summary

ApplicationFlow Cytometry
Sample TestedBlood mononuclear cells (PBMCs)
SpeciesHuman
LotEYT0108031-102616-APC

Comments

CommentsFixed with BD Fix/perm
Intracellular stained with BD perm/wash
Top graph shows stainings with various dilutions in gated monocytes
Bottom graph shows bulk lymphocytes.
Grey filled histogram represents no antibody staining.

FAQs for XIAP Antibody (MAB8221) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional XIAP Products

Blogs on XIAP.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of ...  Read full blog post.

Apoptosis and Necroptosis Part II: Inhibitors of apoptosis proteins (IAPs); Key regulators of the balance between necroptosis, apoptosis and survival
In the first installment of this two-part blog post titled "Apoptosis and Necroptosis: Important factors to identify both types of programmed cell death", the mechanisms by which cell death occurs and ways to identify these pathways were ...  Read full blog post.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and ...  Read full blog post.

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Recent Reviews

3
5
1
4
0
3
0
2
0
1
1

Verified Customer
04/03/2022
Application: IHC-P
Species: Human

Verified Customer
12/11/2016
Application: Flow
Species: Human

Bioinformatics

Gene Symbol XIAP
Uniprot