![Immunocytochemistry/Immunofluorescence: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] - Wnt6 antibody [HL1568] detects Wnt6 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Wnt6 stained by Wnt6 antibody [HL1568] (NBP3-25754) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25754_rabbit-wnt-6-mab-hl1568-azide-and-bsa-free-212024939127.jpg "Immunocytochemistry/Immunofluorescence: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] - Wnt6 antibody [HL1568] detects Wnt6 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Wnt6 stained by Wnt6 antibody [HL1568] (NBP3-25754) diluted at 1:500. Blue: Fluoroshield with DAPI .")
| Reactivity | Hu, Rt, Mu, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1568 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Wnt-6. The exact sequence is proprietary. |
| Predicted Species | Mouse (93%), Bovine (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | WNT6 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Wnt-2b Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Wnt-2b Antibody [Unconjugated]")
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Wnt-6 Antibody (NBP3-25754)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | WNT6 |
![Immunohistochemistry-Paraffin: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] - Wnt6 antibody [HL1568] detects Wnt6 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal gland. Wnt6 stained by Wnt6 antibody [HL1568] (NBP3-25754) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25754_rabbit-wnt-6-mab-hl1568-azide-and-bsa-free-212024940838.jpg "Immunohistochemistry-Paraffin: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] - Wnt6 antibody [HL1568] detects Wnt6 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal gland. Wnt6 stained by Wnt6 antibody [HL1568] (NBP3-25754) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt6 antibody [HL1568] (NBP3-25754) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25754_rabbit-wnt-6-mab-hl1568-azide-and-bsa-free-212024941416.jpg "Western Blot: Wnt-6 Antibody (HL1568) - Azide and BSA Free [NBP3-25754] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt6 antibody [HL1568] (NBP3-25754) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Bioactivity Wnt-5a [Unconjugated]](https://images.novusbio.com/images/protein/645-wn_recombinant-human-mouse-wnt-5a-protein-bioactivity-1612202094911.jpg)
![Bioactivity Wnt-3a [Unconjugated]](https://images.novusbio.com/images2/Wnt3a_5036WN_2880.jpg)
![Bioactivity Wnt-3a [Unconjugated]](https://images.novusbio.com/images2/5036-wn_recombinant-human-wnt-3a-protein-bioactivity-3112023115329.jpg)
![Cell Culture Wnt-3a [Unconjugated]](https://images.novusbio.com/images/5036-wn_recombinant-human-wnt-3a-protein-bioactivity-252023103514.jpg)
![Western Blot Wnt-7b Antibody [Unconjugated]](https://images.novusbio.com/images2/Wnt-7b_AF3460_Western_Blot_11133.jpg)
![Immunohistochemistry Wnt-7b Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af3460_human-wnt-7b-affinity-purified-polyclonal-ab-immunohistochemistry-12122023155533..jpg)
![Western Blot Wnt-7b Antibody [Unconjugated]](https://images.novusbio.com/images/af3460_human-wnt-7b-affinity-purified-polyclonal-ab-41202413392421.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")