Name: Wnt-5a Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF645
Rating: 3.6 (5 reviews)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Mu, RtSpecies Glossary
Applications	WB, IHC
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Biotin BAF645

Catalog# & Formulation	Size	Price
Carrier Free AF645-SP

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF645

Wnt-5a Antibody [Unconjugated]

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Publications for Wnt-5a Antibody (AF645)(55)

Reviews for Wnt-5a Antibody (AF645) (5) 3.65

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Immunogen	E. coli-derived recombinant mouse Wnt‑5a peptide Gln254-Cys334 Accession # P22725
Specificity	Detects mouse and rat Wnt-5a in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Wnt‑5b is observed and less than 2% cross-reactivity with rmWnt-1, rmWnt-3a, rmWnt-4, rmWnt-11, and rmWnt-16 is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	WNT5A
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Publications using AF645	Applications	Species
Ahmad, S;Attisano, L; Wnt5a Promotes Axon Elongation in Coordination with the Wnt-Planar Cell Polarity Pathway Cells 2024-07-28 [PMID: 39120298] (Immunocytochemistry, Mouse)	Immunocytochemistry	Mouse
Mehdawi, LM;Ghatak, S;Chakraborty, P;Sjölander, A;Andersson, T; LGR5 Expression Predicting Poor Prognosis Is Negatively Correlated with WNT5A in Colon Cancer Cells 2023-11-20 [PMID: 37998393] (IHC, Human)	IHC	Human
JR Patel, MZ Joel, KK Lee, A Kambala, H Cornman, O Oladipo, M Taylor, J Deng, V Parthasara, K Cravero, M Marani, R Zhao, S Sankararam, R Li, T Pritchard, V Rebecca, MM Kwatra, W Jin Ho, X Dong, S Kang, SG Kwatra Single-cell RNA sequencing reveals dysregulated fibroblast subclusters in prurigo nodularis bioRxiv : the preprint server for biology, 2023-02-03;0(0):. 2/3/2023 [PMID: 36778229]
CS Thomson, J Pundavela, MR Perrino, RA Coover, K Choi, KE Chaney, TA Rizvi, DA Largaespad, N Ratner WNT5A inhibition alters the malignant peripheral nerve sheath tumor microenvironment and enhances tumor growth Oncogene, 2021-06-02;40(24):4229-4241. 2021-06-02 [PMID: 34079083] (Western Blot, Mouse)	Western Blot	Mouse
AC van Vliet, J Lee, M van der Po, MRJ Mason, JN Noordermee, LG Fradkin, MR Tannemaat, MJA Malessy, J Verhaagen, F De Winter Coordinated changes in the expression of Wnt pathway genes following human and rat peripheral nerve injury PLoS ONE, 2021-04-13;16(4):e0249748. 2021-04-13 [PMID: 33848304] (IHC, Rat)	IHC	Rat
CM Lund, A Dyhl-Polk, DL Nielsen, LB Riis Wnt5a expression and prognosis in stage II-III colon cancer Transl Oncol, 2020-10-09;14(1):100892. 2020-10-09 [PMID: 33045677] (IHC, Human)	IHC	Human
Nibaldo C. Inestrosa, Cheril Tapia-Rojas, Carolina B. Lindsay, Juan Manuel Zolezzi Wnt Signaling Pathway Dysregulation in the Aging Brain: Lessons From the Octodon degus Frontiers in Cell and Developmental Biology 2020-08-05 [PMID: 32850846]
W Kawarazaki, R Mizuno, M Nishimoto, N Ayuzawa, D Hirohama, K Ueda, F Kawakami-M, S Oba, T Marumo, T Fujita Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency J. Clin. Invest., 2020-08-03;0(0):. 2020-08-03 [PMID: 32597829] (Western Blot, Mouse)	Western Blot	Mouse
S Ikezu, H Yeh, JC Delpech, ME Woodbury, AA Van Enoo, Z Ruan, S Sivakumara, Y You, C Holland, T Guillamon-, A Yoshii-Kit, MB Botros, C Madore, PH Chao, A Desani, S Manimaran, SV Kalavai, WE Johnson, O Butovsky, M Medalla, JI Luebke, T Ikezu Inhibition of colony stimulating factor 1 receptor corrects maternal inflammation-induced microglial and synaptic dysfunction and behavioral abnormalities Mol. Psychiatry, 2020-02-18;0(0):. 2020-02-18 [PMID: 32071385] (ELISA Detection, Mouse)	ELISA Detection	Mouse
X Wu, EM van Dijk, JP Ng-Blichfe, IST Bos, C Ciminieri, M Königshoff, LEM Kistemaker, R Gosens Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial Progenitors Cells, 2019-09-25;8(10):. 2019-09-25 [PMID: 31557955] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Chang-Graham AL, Danhof HA, Engevik MA et al. Wnt5a is a TLR2/4-ligand that induces tolerance in human myeloid cells Commun Biol 5/9/2019 [PMID: 31098409]
Levindo Alves de de OLIVEIRA, Celina Tizuko Fujiyama OSHIMA, Pedro Augusto SOFFNER, Marcelo de Souza SILVA, Rodrigo Rego LINS, Andréa Cristina de Moraes MALINVERNI et al. THE CANONICAL WNT PATHWAY IN GASTRIC CARCINOMA ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) 2019-01-07 [PMID: 30624523]
Hui Xiao, Rui Huang, Dai Xiao Cui, Ping Xiao, Mei Diao, Long Li Histopathologic and immunohistochemical findings in congenital anorectal malformations Medicine (Baltimore) 2018-08-05 [PMID: 30075558]
C Han, J Li, C Wang, H Ouyang, X Ding, Y Liu, S Chen, L Luo Wnt5a Contributes to the Differentiation of Human Embryonic Stem Cells into Lentoid Bodies Through the Noncanonical Wnt/JNK Signaling Pathway Invest. Ophthalmol. Vis. Sci., 2018-07-02;59(8):3449-3460. 2018-07-02 [PMID: 30025083] (IHC, Mouse)	IHC	Mouse
K Glaeser, M Urban, E Fenech, O Voloshanen, D Kranz, F Lari, JC Christians, M Boutros ERAD-dependent control of the Wnt secretory factor Evi EMBO J., 2018-01-29;37(4):. 2018-01-29 [PMID: 29378775] (Western Blot, Human)	Western Blot	Human
J Xu, X Li, A Cole, Z Sherman, W Du Reduced Cdc42 Activity Compromises Hematopoiesis-Supportive Function Of Fanconi Anemia Mesenchymal Stromal Cells Stem Cells, 2018-02-09;0(0):. 2018-02-09 [PMID: 29377497] (Western Blot, Mouse)	Western Blot	Mouse
MW Susman, EP Karuna, RC Kunz, TS Gujral, AV Cantú, SS Choi, BY Jong, K Okada, MK Scales, J Hum, LS Hu, MW Kirschner, R Nishinakam, S Yamada, DJ Laird, LE Jao, SP Gygi, ME Greenberg, HH Ho Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates Elife, 2017-09-08;6(0):. 9/8/2017 [PMID: 28885975]
G Huang, S Chubinskay, W Liao, RF Loeser Wnt5a Induces Catabolic Signaling and Matrix Metalloproteinase Production in Human Articular Chondrocytes Osteoarthr. Cartil., 2017-06-03;0(0):. 6/3/2017 [PMID: 28587781]
CM Chen, LL Orefice, SL Chiu, TA LeGates, S Hattar, RL Huganir, H Zhao, B Xu, R Kuruvilla Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice Proc. Natl. Acad. Sci. U.S.A, 2017-01-09;114(4):E619-E628. 2017-01-09 [PMID: 28069946] (Western Blot, Mouse)	Western Blot	Mouse
Hoeke A Baarsma Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD J. Exp. Med, 2016-12-15;0(0):. 2016-12-15 [PMID: 27979969] (Neutralization, Mouse)	Neutralization	Mouse
Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development Sci Rep, 2016-08-26;6(0):32094. 2016-08-26 [PMID: 27561676] (Neutralization, Western Blot, Human, Mouse)	Neutralization, Western Blot	Human, Mouse
Discrete somatic niches coordinate proliferation and migration of primordial germ cells via Wnt signaling J Cell Biol, 2016-07-11;214(2):215-29. 7/11/2016 [PMID: 27402951]
Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation J Biol Chem, 2016-07-11;0(0):. 2016-07-11 [PMID: 27402827] (Bioassay)	Bioassay
IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling PLoS ONE, 2016-05-23;11(5):e0156002. 2016-05-23 [PMID: 27214384] (IHC, Human)	IHC	Human
T Skaria, E Bachli, G Schoedon Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells Cell Adh Migr, 2016-05-09;11(1):24-38. 2016-05-09 [PMID: 27159116] (ICC, Human)	ICC	Human
Bruce F Kimler WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation J. Endocrinol., 2016-03-14;229(2):197-207. 2016-03-14 [PMID: 26975616] (IHC-P, Rat)	IHC-P	Rat
W Wang, N Snyder, A J Worth et al. Regulation of lipid synthesis by the RNA helicase Mov10 controls Wnt5a production. Oncogenesis 1/1/2015 [PMID: 26029828]
Ishizuya-Oka , Atsuko, Kajita , Mitsuko, Hasebe , Takashi Thyroid hormone-regulated Wnt5a/Ror2 signaling is essential for dedifferentiation of larval epithelial cells into adult stem cells in the Xenopus laevis intestine. PLoS ONE, 2014-09-11;9(9):e107611. 2014-09-11 [PMID: 25211363] (Blocking, Western Blot, Xenopus)	Blocking, Western Blot	Xenopus
Kiyoshi Mori, Riko Kitazawa, Takeshi Kondo, Michiko Mori, Yasuhiro Hamada, Michiru Nishida et al. Diabetic Osteopenia by Decreased beta -Catenin Signaling Is Partly Induced by Epigenetic Derepression of sFRP-4 Gene PLoS ONE 2014-07-18 [PMID: 25036934]
Ekstrom E, Bergenfelz C, von Bulow V, Serifler F, Carlemalm E, Jonsson G, Andersson T, Leandersson K WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells. Mol Cancer, 2014-04-26;13(0):88. 2014-04-26 [PMID: 24766647] (Western Blot, Human)	Western Blot	Human
Kumawat K, Menzen M, Slegtenhorst R, Halayko A, Schmidt M, Gosens R TGF-beta-activated kinase 1 (TAK1) signaling regulates TGF-beta-induced WNT-5A expression in airway smooth muscle cells via Sp1 and beta-catenin. PLoS ONE, 2014-04-11;9(4):e94801. 2014-04-11 [PMID: 24728340] (Western Blot, Human)	Western Blot	Human
Dawes L, Sugiyama Y, Lovicu F, Harris C, Shelley E, McAvoy J Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism. Dev Biol, 2013-11-08;385(2):291-303. 2013-11-08 [PMID: 24211762] (Western Blot, Rat)	Western Blot	Rat
Florian M, Nattamai K, Dorr K, Marka G, Uberle B, Vas V, Eckl C, Andra I, Schiemann M, Oostendorp R, Scharffetter-Kochanek K, Kestler H, Zheng Y, Geiger H A canonical to non-canonical Wnt signalling switch in haematopoietic stem-cell ageing. Nature, 2013-10-20;503(7476):392-6. 2013-10-20 [PMID: 24141946] (IHC, Mouse)	IHC	Mouse
Yi-Zhan Xing, Rui-Min Wang, Ke Yang, Hai-Ying Guo, Fang Deng, Yu-Hong Li et al. Adenovirus-Mediated Wnt5a Expression Inhibits the Telogen-To-Anagen Transition of Hair Follicles in Mice International Journal of Medical Sciences 2013-05-21 [PMID: 23781137]
Caroline Bergenfelz, Catharina Medrek, Elin Ekström, Karin Jirström, Helena Janols, Marlene Wullt et al. Wnt5a Induces a Tolerogenic Phenotype of Macrophages in Sepsis and Breast Cancer Patients The Journal of Immunology 2012-06-01 [PMID: 22547701]
Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex. J. Cell. Sci., 2012-02-10;125(0):2017-29. 2012-02-10 [PMID: 22328498] (Western Blot, Mouse)	Western Blot	Mouse
NMDA receptor activation stimulates transcription-independent rapid wnt5a protein synthesis via the MAPK signaling pathway. Mol Brain, 2012-01-04;5(0):1. 2012-01-04 [PMID: 22217152] (ICC, Western Blot, Mouse)	ICC, Western Blot	Mouse
Diana J. Laird, Svetlana Altshuler-Keylin, Michael D. Kissner, Xin Zhou, Kathryn V. Anderson Ror2 Enhances Polarity and Directional Migration of Primordial Germ Cells PLoS Genetics 2011-12-22 [PMID: 22216013]
Hong-Xiang Liu, Ann S. Grosse, Ken Iwatsuki, Yuji Mishina, Deborah L. Gumucio, Charlotte M. Mistretta Separate and distinctive roles for Wnt5a in tongue, lingual tissue and taste papilla development Developmental Biology 2012-01-01 [PMID: 22024319]
Li B, Zhong L, Yang X, Andersson T, Huang M, Tang SJ WNT5A Signaling Contributes to Abeta-Induced Neuroinflammation and Neurotoxicity. PLoS ONE, 2011-08-17;6(8):e22920. 2011-08-17 [PMID: 21857966] (IHC-P, Western Blot, Mouse)	IHC-P, Western Blot	Mouse
Bitler BG, Nicodemus JP, Li H, Cai Q, Wu H, Hua X, Li T, Birrer MJ, Godwin AK, Cairns P, Zhang R Wnt5a suppresses epithelial ovarian cancer by promoting cellular senescence. Cancer Res., 2011-08-04;71(19):6184-94. 2011-08-04 [PMID: 21816908] (IHC, Western Blot, Human)	IHC, Western Blot	Human
Plasilova M, Chattopadhyay C, Ghosh A et al. Discordant Gene Expression Signatures and Related Phenotypic Differences in Lamin A-and A/C-Related Hutchinson-Gilford Progeria Syndrome (HGPS). PLoS One. 6/27/2011 [PMID: 21738662]
Sha Peng, Junlin Zhang, Jiahuan Chen, Huayan Wang Effects of Wnt5a protein on proliferation and apoptosis in JAR choriocarcinoma cells Molecular Medicine Reports 2010-10-19 [PMID: 21461571]
Qin Wang, Andrew J. Symes, Corrina A. Kane, Alex Freeman, Joseph Nariculam, Philippa Munson et al. A Novel Role for Wnt/Ca2+ Signaling in Actin Cytoskeleton Remodeling and Cell Motility in Prostate Cancer PLoS ONE 2010-05-04 [PMID: 20454608]
Gudjonsson JE, Johnston A, Stoll SW Evidence for altered Wnt signaling in psoriatic skin. J. Invest. Dermatol., 2010-04-08;130(7):1849-59. 2010-04-08 [PMID: 20376066] (Bioassay, IHC-P, Human)	Bioassay, IHC-P	Human
Bodmer D, Levine-Wilkinson S, Richmond A, Hirsh S, Kuruvilla R Wnt5a mediates nerve growth factor-dependent axonal branching and growth in developing sympathetic neurons. J. Neurosci., 2009-06-10;29(23):7569-81. 2009-06-10 [PMID: 19515925] (ICC, Western Blot, Mouse)	ICC, Western Blot	Mouse
Christman MA, Goetz DJ, Dickerson E, McCall KD, Lewis CJ, Benencia F, Silver MJ, Kohn LD, Malgor R Wnt5a is expressed in murine and human atherosclerotic lesions. Am. J. Physiol. Heart Circ. Physiol., 2008-05-02;294(6):H2864-70. 2008-05-02 [PMID: 18456733] (IHC-P, Human)	IHC-P	Human
Pereira C, Schaer DJ, Bachli EB, Kurrer MO, Schoedon G Wnt5A/CaMKII signaling contributes to the inflammatory response of macrophages and is a target for the antiinflammatory action of activated protein C and interleukin-10. Arterioscler. Thromb. Vasc. Biol., 2008-01-03;28(3):504-10. 2008-01-03 [PMID: 18174455] (ICC, Human)	ICC	Human
Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G Dickkopf-1 is a master regulator of joint remodeling. Nat. Med., 2007-01-21;13(2):156-63. 2007-01-21 [PMID: 17237793] (IHC-P, Western Blot, Human, Mouse)	IHC-P, Western Blot	Human, Mouse
Masckauchan TN, Agalliu D, Vorontchikhina M, Ahn A, Parmalee NL, Li CM, Khoo A, Tycko B, Brown AM, Kitajewski J Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2. Mol. Biol. Cell, 2006-10-11;17(12):5163-72. 2006-10-11 [PMID: 17035633] (IHC, Mouse)	IHC	Mouse
Blumenthal A, Ehlers S, Lauber J, Buer J, Lange C, Goldmann T, Heine H, Brandt E, Reiling N The Wingless homolog WNT5A and its receptor Frizzled-5 regulate inflammatory responses of human mononuclear cells induced by microbial stimulation. Blood, 2006-04-06;108(3):965-73. 2006-04-06 [PMID: 16601243] (Neutralization, Human)	Neutralization	Human
Castelo-Branco G, Sousa KM, Bryja V, Pinto L, Wagner J, Arenas E Ventral midbrain glia express region-specific transcription factors and regulate dopaminergic neurogenesis through Wnt-5a secretion. Mol. Cell. Neurosci., 2005-10-21;31(2):251-62. 2005-10-21 [PMID: 16243537] (ICC, Mouse)	ICC	Mouse
Polesskaya A, Seale P, Rudnicki MA Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration. Cell, 2003-06-27;113(7):841-52. 2003-06-27 [PMID: 12837243] (IHC, Mouse)	IHC	Mouse
Murdoch B, Chadwick K, Martin M, Shojaei F, Shah KV, Gallacher L, Moon RT, Bhatia M Wnt-5A augments repopulating capacity and primitive hematopoietic development of human blood stem cells in vivo. Proc. Natl. Acad. Sci. U.S.A., 2003-03-07;100(6):3422-7. 2003-03-07 [PMID: 12626754] (Immunodepletion, Xenopus)	Immunodepletion	Xenopus
Ahumada A, Slusarski DC, Liu X, Moon RT, Malbon CC, Wang HY Signaling of rat Frizzled-2 through phosphodiesterase and cyclic GMP. Science, 2002-12-06;298(5600):2006-10. 2002-12-06 [PMID: 12471263] (Immunodepletion, Mouse)	Immunodepletion	Mouse
Show All 55 Publications. Collapse Publications.

	Verified Customer 01/09/2015
Application:	WB
Species:	Human

	Verified Customer 01/09/2015
Application:	IHC-P
Species:	Human

	Verified Customer 01/09/2015
Application:	IHC-Fr
Species:	Mouse

Gene Symbol	WNT5A
Uniprot	P22725()