![Western Blot: Wnt-5a Antibody (S08-4A3) [NBP3-20061] - Western blot detection of Wnt-5a in Hela cell lysates using NBP3-20061 (1:500 diluted). Predicted band size: 42kDa. Observed band size: 45kDa.](http://images.novusbio.com/fullsize/Wnt-5a-Antibody-S08-4A3-Western-Blot-NBP3-20061-img0002.jpg "Western Blot: Wnt-5a Antibody (S08-4A3) [NBP3-20061] - Western blot detection of Wnt-5a in Hela cell lysates using NBP3-20061 (1:500 diluted). Predicted band size: 42kDa. Observed band size: 45kDa.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | S08-4A3 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.3 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide of human Wnt-5a (Uniprot # P41221) |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | WNT5A |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA |
| Preservative | 0.01% Sodium Azide |
| Concentration | 0.3 mg/ml |
| Purity | Affinity purified |
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Wnt-5a Antibody (NBP3-20061)Find related products by research area.
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The role of Wnts in neuroinflammation By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | WNT5A |
![Immunocytochemistry/Immunofluorescence: Wnt-5a Antibody (S08-4A3) [NBP3-20061] - Immunocytochemistry of Wnt-5a (green) in hela using NBP3-20061 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/Wnt-5a-Antibody-S08-4A3-Immunocytochemistry-Immunofluorescence-NBP3-20061-img0001.jpg "Immunocytochemistry/Immunofluorescence: Wnt-5a Antibody (S08-4A3) [NBP3-20061] - Immunocytochemistry of Wnt-5a (green) in hela using NBP3-20061 at dilution 1/50, and DAPI (blue)")
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![Cell Culture Dkk-1 [Unconjugated]](https://images.novusbio.com/images/5439-dk_recombinant-human-dkk-1-protein-cell-culture-105202416211..jpg)
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![Immunocytochemistry JNK1 Antibody (228601) [Unconjugated]](https://images.novusbio.com/images2/JNK1_MAB17761_Immunocytochemistry__Immunofluorescence_20184.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")