![Western Blot: WIPI1 Overexpression Lysate (Adult Normal) [NBL1-17853] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WIPI1.](http://images.novusbio.com/fullsize/WIPI1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17853-img0002.jpg "Western Blot: WIPI1 Overexpression Lysate (Adult Normal) [NBL1-17853] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WIPI1.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Description | WIPI1 Transient Overexpression LysateExpression Host: HEK293T Plasmid: RC208094 Accession#: NM_017983 Protein Tag: C-MYC/DDK You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT. |
| Gene | WIPI1 |
| Dilutions |
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| Application Notes | This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading. |
| Theoretical MW | 48.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | RIPA buffer |
Research Areas for WIPI1 Lysate (NBL1-17853)Find related products by research area.
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Epigenetic Control of Autophagy By Christina Towers, PhD. In the last 20 years, epigenetic regulation has become front and center for almost all fields of biology and its role in diseases like cancer and neurodegeneration are being heavily studi... Read full blog post. |
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WIPI1 - An essential regulator of early autophagosome assembly WD repeat domain phosphoinositide-interacting protein 1 (WIPI) is involved in the lysosomal degradation of cytoplasmic components during starvation-induced autophagy. WIPI1 is a seven bladed beta-propeller protein that provides a scaffold for the ... Read full blog post. |
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| Gene Symbol | WIPI1 |
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