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VPS25 Antibody (2E5-2B9)

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Western Blot: VPS25 Antibody (2E5-2B9) [H00084313-M01] - MGC10540 monoclonal antibody (M01), clone 2E5-2B9 Analysis of MGC10540 expression in Hela S3 NE.
Western Blot: VPS25 Antibody (2E5-2B9) [H00084313-M01] - MGC10540 monoclonal antibody (M01), clone 2E5-2B9 Analysis of MGC10540 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: VPS25 Antibody (2E5-2B9) [H00084313-M01] - Analysis of monoclonal antibody to VPS25 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: VPS25 Antibody (2E5-2B9) [H00084313-M01] - Detection limit for recombinant GST tagged VPS25 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
2E5-2B9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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VPS25 Antibody (2E5-2B9) Summary

Immunogen
MGC10540 (AAH06282, 1 a.a. ~ 176 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLPVESIQIVLEELRKKGNLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDEEFHGLDEATLLRALQALQQEHKAEIITVSDGRGVKFF
Specificity
VPS25 - hypothetical protein MGC10540
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
VPS25
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for VPS25 Antibody (2E5-2B9)

  • Dermal papilla-derived protein 9
  • DERP9
  • DERP9hVps25
  • EAP20
  • EAP20FAP20
  • ELL-associated protein of 20 kDa
  • ESCRT-II complex subunit VPS25
  • FAP20
  • MGC10540
  • vacuolar protein sorting 25 (yeast)
  • vacuolar protein sorting 25 homolog (S. cerevisiae)
  • vacuolar protein-sorting-associated protein 25

Background

VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00084313-M01
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Bioinformatics

Gene Symbol VPS25
Entrez
Uniprot