![Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412] - (4ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/VPS11-Antibody-Immunohistochemistry-Paraffin-NB100-2412-img0002.jpg "Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412] - (4ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.")
| Reactivity | Hu, Mu, Rt, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-HALQEKDTQTKLE corresponding to internal region according to NP_068375.3. |
| Predicted Species | Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | VPS11 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Preliminary experiments gave an approx. 75 kDa band in human duodenum, colon and placenta lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 108 kDa band according to NP_068375.3. The 75 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows brushborder staning in PCT cells and human pancreas shows cytoplasm staining of beta cells in islets of langerhans. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NB100-2412 | Applications | Species |
|---|---|---|
| Kim BY, Kramer H, Yamamoto A et al. Molecular characterization of mammalian homologues of class C Vps proteins that interact with syntaxin-7. J Biol Chem 2001-08-03 [PMID: 11382755] |
Secondary Antibodies |
Isotype Controls |
Research Areas for VPS11 Antibody (NB100-2412)Find related products by research area.
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| Gene Symbol | VPS11 |
| Entrez | |
| Uniprot |
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![Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412] - 3.8ug/ml staining of paraffin embedded Human Kidney-. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/VPS11-Antibody-Immunohistochemistry-Paraffin-NB100-2412-img0001.jpg "Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412] - 3.8ug/ml staining of paraffin embedded Human Kidney-. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
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