Home » VGF » VGF Antibodies » VGF Antibody

VGF Antibody

 Submit a Review

Images

 
(
Enlarge)
Immunocytochemistry/Immunofluorescence: VGF Antibody [NBP2-31596] - Staining of human cell line SK-MEL-30 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: VGF Antibody [NBP2-31596] - Staining of human cell line SK-MEL-30 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human pituitary gland shows strong cytoplasmic positivity in anterior cells.
Orthogonal Strategies: Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining in human cerebral cortex and liver tissues using anti-VGF antibody. Corresponding VGF RNA-seq data are presented for the ...read more
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human colon.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human testis.
Orthogonal Strategies: Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Analysis in human cerebral cortex and prostate tissues. Corresponding VGF RNA-seq data are presented for the same tissues.
Independent Antibodies: Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human hypothalamus, pituitary gland, placenta and prostate using Anti-VGF antibody NBP2-31596 (A) shows similar ...read more
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human hypothalamus shows strong positivity in a subset of neurons.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human pituitary gland shows strong positivity in anterior cells.
Immunohistochemistry-Paraffin: VGF Antibody [NBP2-31596] - Staining of human placenta shows no positivity in trophoblastic cells as expected.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

      

Orthogonal Strategies

  

Order Details

Novus Biologicals offers 100% Guarantee

VGF Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: APARDELPDWNEVLPPWDREEDEVYPPGPYHPFPNYIRPRTLQPPSALRRRHYHHALPPSRHYPGREAQARRAQEEAEAEERRLQEQEELENYIEHV
Predicted Species
Mouse (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VGF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
VGF Recombinant Protein Antigen (NBP2-31596PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for VGF Antibody

  • neuro-endocrine specific protein VGF
  • neurosecretory protein VGF
  • VGF nerve growth factor inducible
  • VGF

Background

Nerve growth factor (NGF) is a peptide that plays a key role in the differentiation and survival of neurons in the peripheral nervous system (PNS) and the central nervous system (CNS). VGF is a peptide synthesized and secreted by neurons and is upregulated by NGF in the PC12 cell line. VGF is widely expressed in both the PNS and CNS, but is especially abundant in the adult hypothalamus. VGF plays an essential role in how the brain regulates energy expenditure and body weight. Its expression is rapidly induced by injury, the circadian clock, and neuronal activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

BDNF [HRP]
DBD00
N/A BDNF [HRP]
N/A BDNF [HRP]
N/A BDNF [HRP]
(104 Publications)
Species: Hu
Applications: ELISA
EGF [Unconjugated]
236-EG
SDS-PAGE EGF [Unconjugated]
Bioactivity EGF [Unconjugated]
Cell Culture EGF [Unconjugated]
(861 Publications)
Species: Hu
Applications: BA
beta-NGF
[Unconjugated]
256-GF
SDS-PAGE beta-NGF [Unconjugated]
Bioactivity beta-NGF [Unconjugated]
(60 Publications)
Species: Hu
Applications: BA
Neuropeptide Y
Antibody
NBP1-46535
Immunohistochemistry Neuropeptide Y Antibody
Immunocytochemistry/ Immunofluorescence Neuropeptide Y Antibody
(23 Publications)
Species: Hu, Mu, Rb, Rt, Xp
Applications: ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
EGFR Antibody
[Unconjugated]
AF231
Immunohistochemistry EGFR Antibody [Unconjugated]
Immunocytochemistry EGFR Antibody [Unconjugated]
Western Blot EGFR Antibody [Unconjugated]
(30 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Chromogranin C
Antibody
NBP2-15895
Immunohistochemistry-Paraffin Chromogranin C Antibody
Western Blot Chromogranin C Antibody
Western Blot Chromogranin C Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
PCSK1N Antibody
NBP1-80797
Immunohistochemistry-Paraffin PCSK1N Antibody
Immunohistochemistry-Paraffin PCSK1N Antibody
Immunohistochemistry-Paraffin PCSK1N Antibody
(1 Publication)
Species: Hu
Applications: IHC, IHC-P
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(685 Publications)
Species: Hu
Applications: ELISA
GM-CSF
[Unconjugated]
7954-GM/CF
Bioactivity GM-CSF [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
LAMC2 Antibody
(CL2980)
NBP2-42388
Western Blot LAMC2 Antibody (CL2980)
Immunohistochemistry-Paraffin LAMC2 Antibody (CL2980)
Immunohistochemistry-Paraffin LAMC2 Antibody (CL2980)
(4 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
EGR1 Antibody
[Unconjugated]
AF2818
Immunocytochemistry EGR1 Antibody [Unconjugated]
(13 Publications)
Species: Hu
Applications: ICC, WB
Proprotein
Convertase 1/PCSK1 An ...
H00005122-M02
Immunocytochemistry/ Immunofluorescence Proprotein Convertase 1/PCSK1 Antibody (3D2)
Immunohistochemistry-Paraffin Proprotein Convertase 1/PCSK1 Antibody (3D2)
Sandwich ELISA Proprotein Convertase 1/PCSK1 Antibody (3D2)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
Chromogranin B
Antibody
NBP1-80782
Immunohistochemistry-Paraffin Chromogranin B Antibody
Immunohistochemistry-Paraffin Chromogranin B Antibody
Immunohistochemistry-Paraffin Chromogranin B Antibody
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
POMC Antibody
NB100-1533
Flow Cytometry POMC Antibody
Immunohistochemistry POMC Antibody
Immunohistochemistry POMC Antibody
(11 Publications)
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
Chromogranin A
Antibody - BSA Free
NB120-15160
Immunocytochemistry/ Immunofluorescence Chromogranin A Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Chromogranin A Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Chromogranin A Antibody - BSA Free
(2 Reviews)
(43 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Ret Antibody
[Unconjugated]
AF482
Immunohistochemistry Ret Antibody [Unconjugated]
Western Blot Ret Antibody [Unconjugated]
Immunohistochemistry Ret Antibody [Unconjugated]
(40 Publications)
Species: Mu
Applications: IHC, WB
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(25 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
VGF Antibody
NBP2-31596
VGF Antibody
Species: Hu, Mu
Applications: ICC/IF, IHC

Publications for VGF Antibody (NBP2-31596) (0)

There are no publications for VGF Antibody (NBP2-31596).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for VGF Antibody (NBP2-31596) (0)

There are no reviews for VGF Antibody (NBP2-31596). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for VGF Antibody (NBP2-31596) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional VGF Products

Research Areas for VGF Antibody (NBP2-31596)

Find related products by research area.

Blogs on VGF

There are no specific blogs for VGF, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our VGF Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol VGF
Uniprot