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VEGFR1/Flt-1 Antibody (SY09-09)

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Western Blot: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Western blot analysis of VEGFR1/Flt-1 on mouse lung lysates using NBP3-11864 at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - ICC staining VEGFR1/Flt-1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using NBP3-11864. Counter stained with hematoxylin.
Flow Cytometry: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Flow cytometric analysis of A431 cells with NBP3-11864 at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary ...read more
Immunocytochemistry/ Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunocytochemistry staining VEGFR1/Flt-1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in ...read more
Immunocytochemistry/ Immunofluorescence: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunocytochemistry staining VEGFR1/Flt-1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in ...read more
Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody (SY09-09) [NBP3-11864] - Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using NBP3-11864. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, ICC/IF
Clone
SY09-09
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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VEGFR1/Flt-1 Antibody (SY09-09) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein (Uniprot#: P17948)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
FLT1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:1000 - 1:2000
Theoretical MW
151 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
1X TBS (pH7.4), 1% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for VEGFR1/Flt-1 Antibody (SY09-09)

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT
  • FLT1
  • Flt-1
  • Fms-like tyrosine kinase 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
  • FRT
  • Tyrosine-protein kinase FRT
  • Tyrosine-protein kinase receptor FLT
  • vascular endothelial growth factor receptor 1
  • Vascular permeability factor receptor
  • VEGF R1
  • VEGFR1
  • VEGFR-1

Background

Receptors for VEGF, VEGFB and PGF have tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. VEGF and its high-affinity binding receptors--the tyrosine kinases FLK1 and FLT1--are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol FLT1