VEGF-B Antibody (OTI1H9)

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Western Blot: VEGF-B Antibody (OTI1H9) [NBP2-46330] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGF-B.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1H9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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Support & Research

VEGF-B Antibody (OTI1H9) Summary

Immunogen
Full length human recombinant protein of human VEGFB(NP_003368) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VEGFB
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:4000
Theoretical MW
21.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VEGF-B Antibody (OTI1H9)

  • vascular endothelial growth factor B
  • VEGFB
  • VEGF-B
  • VEGF-related factor
  • VRFVEGFL

Background

VEGFB 186 is an isoform of VEGFB (Vascular Endothelial Growth Factor B), a member of the vascular endothelial growth factor (VEGF) family. It is a growth factor for endothelial cells. Both isoforms of VEGFB show strong homology to VEGF at their amino termini, wheras the VEGFB167 isoform binds heparin, the C terminus of VEGFB-186 has no heparin binding domain, is less basic and more hydrophobic, and has no similarity to any published sequence. VEGFB 186 is secreted as a disulfide-linked homodimer and that, like VEGFB 167, this isoform can form disulfide-linked heterodimers with VEGF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol VEGFB