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VAP-1/AOC3 Antibody

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Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody [NB100-57090] - (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody [NB100-57090] - (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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VAP-1/AOC3 Antibody Summary

Immunogen
Peptide with sequence C-DEDPSFYSADSIY corresponding to internal region (near C-Terminus) according to NP_003725.1.
Epitope
DEDPSFYSADSIY
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AOC3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Preliminary experiments gave an approx. 55 kDa band in human adipose and lung lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 84.6 kDa band according to NP_003725.1. The 55 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human liver shows membranous staining in sinusoid lining cells surrounding hepatocytes.
Reviewed Applications
Read 1 Review rated 4
using
NB100-57090 in the following applications:
Publications
Read Publication using NB100-57090.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VAP-1/AOC3 Antibody

  • amine oxidase, copper containing 3 (vascular adhesion protein 1)
  • AOC3
  • Copper amine oxidase
  • EC 1.4.3
  • HPAO
  • HPAOSSAO
  • Semicarbazide-sensitive amine oxidase
  • SSAO
  • VAP1
  • VAP-1
  • VAP1EC 1.4.3.21
  • VAP-1membrane primary amine oxidase
  • Vascular adhesion protein 1

Background

Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The product is a major protein on the adipocyte plasma membrane. It has adhesive properties and also has functional monoamine oxidase activity. A pseudogene for this gene has been described and is located approximately 9-kb downstream.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VAP-1/AOC3 Antibody (NB100-57090)(1)


Review for VAP-1/AOC3 Antibody (NB100-57090) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-57090:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot VAP-1/AOC3 NB100-57090
Enlarge 		4
reviewed by:
Verified Customer
 WB Human 01/07/2015
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Summary

ApplicationWestern Blot
SpeciesHuman
LotP1

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Secondary Antibodies

 

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Recent Reviews

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Western Blot VAP-1/AOC3 NB100-57090
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 Verified Customer
01/07/2015
Application: WB
Species: Human
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Bioinformatics

Gene Symbol AOC3
Entrez
Uniprot