![Immunocytochemistry/Immunofluorescence: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.](http://images.novusbio.com/fullsize/UFM1-Activating-Enzyme-UBA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82087-img0004.jpg "Immunocytochemistry/Immunofluorescence: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: GIGKLLLFDYDKVELANMNRLFFQPHQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPVDLVLSCVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANIDEKTL |
| Predicted Species | Mouse (94%), Rat (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | UBA5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | UBA5 |
![Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/UFM1-Activating-Enzyme-UBA5-Antibody-Immunohistochemistry-Paraffin-NBP1-82087-img0007.jpg "Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.")
![Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/UFM1-Activating-Enzyme-UBA5-Antibody-Immunohistochemistry-Paraffin-NBP1-82087-img0002.jpg "Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/UFM1-Activating-Enzyme-UBA5-Antibody-Immunohistochemistry-Paraffin-NBP1-82087-img0005.jpg "Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.")
![Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087] - Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/UFM1-Activating-Enzyme-UBA5-Antibody-Immunohistochemistry-Paraffin-NBP1-82087-img0006.jpg "Immunohistochemistry-Paraffin: UFM1 Activating Enzyme/UBA5 Antibody [NBP1-82087] - Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.")
![Western Blot Butyrylcholinesterase/BCHE Antibody [Unconjugated]](https://images.novusbio.com/images2/Butyrylcholinesterase_AF9024_Western_Blot_19121.jpg)
![Immunohistochemistry Butyrylcholinesterase/BCHE Antibody [Unconjugated]](https://images.novusbio.com/images2/Butyrylcholinesterase_AF9024_Immunohistochemistry_19158.jpg)
![Ubiquitin-activating Enzyme/UBE1 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Ubiquitin-activating Enzyme/UBE1 [Unconjugated]")
![N/A Erythropoietin/EPO [HRP]](https://images.novusbio.com/images2/DATA_Erythropoietin_MEP00_ELISA_970.jpg)
![N/A Erythropoietin/EPO [HRP]](https://images.novusbio.com/images2/Erythropoietin_MEP00_ELISA_421.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")