![Western Blot: UCP1 Antibody [NBP2-20796] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/UCP1-Antibody-Western-Blot-NBP2-20796-img0008.jpg "Western Blot: UCP1 Antibody [NBP2-20796] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human UCP1. The exact sequence is proprietary. |
| Localization | Mitochondrion inner membrane; Multi-pass membrane protein |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | UCP1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in KO reported in scientific publication (PMID: 32713230). |
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| Theoretical MW | 33 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for UCP1 Antibody (NBP2-20796)Find related products by research area.
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![Immunohistochemistry-Paraffin: UCP1 Antibody [NBP2-20796] - Mouse brown adipocyte. UCP1 stained by UCP1 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/UCP1-Antibody-Immunohistochemistry-Paraffin-NBP2-20796-img0006.jpg "Immunohistochemistry-Paraffin: UCP1 Antibody [NBP2-20796] - Mouse brown adipocyte. UCP1 stained by UCP1 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: UCP1 Antibody [NBP2-20796] - Human colon carcinoma, using UCP1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/UCP1-Antibody-Immunohistochemistry-Paraffin-NBP2-20796-img0003.jpg "Immunohistochemistry-Paraffin: UCP1 Antibody [NBP2-20796] - Human colon carcinoma, using UCP1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: UCP1 Antibody [NBP2-20796] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody (NBP2-20796) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20796_rabbit-polyclonal-ucp1-antibody-910202420115033.jpg "Western Blot: UCP1 Antibody [NBP2-20796] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody (NBP2-20796) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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