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UBTF Antibody (1A2)

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Western Blot: UBTF Antibody (1A2) [H00007343-M03] - Analysis of UBTF expression in PC-12 (Cat # L012V1).
Immunocytochemistry/ Immunofluorescence: UBTF Antibody (1A2) [H00007343-M03] - Analysis of monoclonal antibody to UBTF on HepG2 cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: UBTF Antibody (1A2) [H00007343-M03] - Analysis of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. Antibody concentration 0.5 ug/ml
Western Blot: UBTF Antibody (1A2) [H00007343-M03] - Analysis of UBTF expression in HepG2 (Cat # L019V1).
ELISA: UBTF Antibody (1A2) [H00007343-M03] - Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
1A2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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UBTF Antibody (1A2) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
UBTF (NP_055048, 551 a.a. ~ 650 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYIS
Specificity
UBTF (1A2)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
UBTF
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UBTF Antibody (1A2)

  • 90-KDa Nucleolus Organizer Region Autoantigen
  • Autoantigen NOR-90
  • NOR-90
  • nucleolar transcription factor 1
  • UBF
  • UBF1
  • UBF-1
  • UBF2
  • UBFUBF-1
  • upstream binding transcription factor, RNA polymerase I
  • Upstream-binding factor 1

Background

Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol UBTF
Entrez
OMIM
Uniprot