TXNIP Antibody (JY2) - Azide and BSA Free

Vitamin D3 up-regulated protein 1 (VDUP-1), also called Thioredoxin (TRX)-interacting protein, is an endogenous inhibitor of TRX. Redox-dependent regulation of VDUP-1 by mitogenic factors through Reactive oxygen species (ROS) and the specific binding of VDUP-1 to the redox-sensitive cysteine-sulfide center of TRX modulate intracellular levels of ROS and the mitogenic activity of TRX. It has been reported that TXNIP plays important roles in diverse cellular processes, including the regulation of cellular redox balance, apoptosis, proliferation, and differentiation.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Amphibian.

We have publications tested in 11 applications: IF/IHC, IHC-P, IP, Immunohistochemistry-Paraffin, Immunoprecipitation, KD, Knockout Validated, PLA, Simple Western, WB, Western Blot.

Showing Publications 1 - 10 of 38. Show All 38 Publications. Collapse Publications.

Publications using NBP1-54578	Applications	Species
Patrick Devlin Inflammatory Response Following Hemorrhagic Stroke: The Role of Cytokines University of Tennessee Health Science Center 2022-11-07 (WB, Mouse)	WB	Mouse
Kim, J;Lim, J;Yoo, ID;Park, S;Moon, JS; TXNIP contributes to induction of pro-inflammatory phenotype and caspase-3 activation in astrocytes during Alzheimer's diseases Redox biology 2023-05-06 [PMID: 37172394] (Immunohistochemistry-Paraffin, Human)	Immunohistochemistry-Paraffin	Human
Tsubaki H, Mendsaikhan A, Buyandelger U et al. Localization of Thioredoxin-Interacting Protein in Aging and Alzheimer's Disease Brains NeuroSci 2022-03-31 (IF/IHC, WB, Human)	IF/IHC, WB	Human
Brocker CN, Kim D, Melia T et al. Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to metabolic stress bioRxiv (WB, Knockout Validated, Mouse)	WB, Knockout Validated	Mouse
Singh G, Storey KB TXNIP shuttling - a key molecular link in regulating inflammation and mitochondrial dysfunction in freeze tolerant wood frogs Gene 2023-01-07 [PMID: 36627089] (WB, Amphibian)	WB	Amphibian
Ismael S, Patrick D, Salman M et al. Verapamil inhibits TXNIP-NLRP3 inflammasome activation and preserves functional recovery after intracerebral hemorrhage in mice Neurochemistry international 2022-10-14 [PMID: 36244583] (WB, IF/IHC, Mouse) Details: Dilution used in WB 1:1000, in IHC 1:100	WB, IF/IHC	Mouse
Girdhar K, Thakur S, Gaur P et al. Design, synthesis, and biological evaluation of a small molecule oral agonist of the glucagon-like-peptide-1 receptor Journal of Biological Chemistry 2022-04-01 [PMID: 35378127] (WB, Mouse)	WB	Mouse
Salman M, Ismael S, Lexiao L Et al. Acute Hyperglycemia Exacerbates Hemorrhagic Transformation after Embolic Stroke and Reperfusion with tPA: A Possible Role of TXNIP-NLRP3 Inflammasome Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 2021-11-27 [PMID: 34847489] (WB, Mouse)	WB	Mouse
Singh G, Storey KB MondoA:MLX complex regulates glucose-dependent gene expression and links to circadian rhythm in liver and brain of the freeze-tolerant wood frog, Rana sylvatica Mol. Cell. Biochem. 2020-07-07 [PMID: 32638259]
Ko JW, Shin NR, Je-Oh L et al. Silica dioxide nanoparticles aggravate airway inflammation in an asthmatic mouse model via NLRP3 inflammasome activation Regul. Toxicol. Pharmacol. 2020-02-19 [PMID: 32087352] (IF/IHC, Mouse) Details: BALB/c mice	IF/IHC	Mouse
Singh G Role of Glucose-induced Transcription Factor Signalling and Mitochondrial Epigenetics in Stress Tolerant Wood Frog, Rana sylvatica Thesis 2022-12-16 (Immunoprecipitation, Western Blot, Amphibian) Details: Wood Frog	Immunoprecipitation, Western Blot	Amphibian
Lim JO, Lee SJ, Kim WI et al. Melatonin Alleviates Silica Nanoparticle-Induced Lung Inflammation via Thioredoxin-Interacting Protein Downregulation Antioxidants (Basel) 2021-11-04 [PMID: 34829636]
Salman M, Ismael S, Li L Et al. Endothelial Thioredoxin-Interacting Protein Depletion Reduces Hemorrhagic Transformation in Hyperglycemic Mice after Embolic Stroke and Thrombolytic Therapy Pharmaceuticals (Basel, Switzerland) 2021-09-27 [PMID: 34681207] (WB, Mouse)	WB	Mouse
Yin H, Wang K, Das A et al. The REDD1/TXNIP Complex Accelerates Oxidative Stress-Induced Apoptosis of Nucleus Pulposus Cells through the Mitochondrial Pathway Oxidative Medicine and Cellular Longevity 2021-09-22 [PMID: 34603601] (IP, Human)	IP	Human
Lim J, Lee S, Kim W Et al. Titanium dioxide nanoparticles exacerbate allergic airway inflammation via TXNIP upregulation in a mouse model of asthma Research Square 2021-08-03 [PMID: 34576095] (IHC-P, WB, Mouse)	IHC-P, WB	Mouse
Chen YS, Weng SJ, Chang SH Et al. Evaluating the antidiabetic effects of R-verapamil in type 1 and type 2 diabetes mellitus mouse models PloS one 2021-08-06 [PMID: 34358247] (Simple Western, Mouse)	Simple Western	Mouse
Ismael S, Nasoohi S, Yoo A et al. Verapamil as an Adjunct Therapy to Reduce tPA Toxicity in Hyperglycemic Stroke: Implication of TXNIP/NLRP3 Inflammasome Molecular Neurobiology 2021-08-01 [PMID: 33847912]
Ismael S, Nasoohi S, Yoo A et al. Tissue Plasminogen Activator Promotes TXNIP-NLRP3 Inflammasome Activation after Hyperglycemic Stroke in Mice Molecular Neurobiology 2020-06-01 [PMID: 32172516]
Bronner DN. Cells on Fire: Regulation of the NLRP3 inflammasome via endoplasmic reticulum and mitochondrial crosstalk. Thesis. 2015-01-01 (WB, Mouse)	WB	Mouse
Brocker CN, Kim D, Melia T et al. Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to PPARA agonism and fasting Nat Commun 2020-11-17 [PMID: 33203882] (WB, Mouse) Details: Mouse primary hepatocytes and NIHT3T cells transfected with Gm15441 and TXNIP plasmids underwent Western blot analysis.	WB	Mouse
Wei H, Bu R, Yang Q Exendin-4 Protects against Hyperglycemia-Induced Cardiomyocyte Pyroptosis via the AMPK-TXNIP Pathway J Diabetes Res 2019-12-09 [PMID: 31886288] (KD, WB, Mouse)	KD, WB	Mouse
Macer-Wright JL, Sileikaite I, Rayner BS, Hawkins CL 8-chloroadenosine alters the metabolic profile and downregulates antioxidant and DNA damage repair pathways in macrophages Chem. Res. Toxicol. 2019-11-28 [PMID: 31778309]
Yuan J, Tan JT, Rajamani K et al. Fenofibrate Rescues Diabetes-Related Impairment of Ischemia-Mediated Angiogenesis by PPARa-Independent Modulation of Thioredoxin Interacting Protein Diabetes 2019-02-14 [PMID: 30765336] (WB, Mouse)	WB	Mouse
Zaragoza-Campillo MA, Moran J. Reactive oxygen species evoked by potassium deprivation and staurosporine inactivate Akt and induce the expression of TXNIP in cerebellar granule neurons. Oxidative Medicine and Cellular Longevity. 2017-01-22 [PMID: 28367274] (WB, Rat)	WB	Rat
Chen W, Wang J, Luo Y et al. Ginsenoside Rb1 and compound K improve insulin signaling and inhibit ER stress-associated NLRP3 inflammasome activation in adipose tissue. J Ginseng Res 2016-10-01 [PMID: 27746687]
Ahrens TD, Timme S, Ostendorp J et al. Response of esophageal cancer cells to epigenetic inhibitors is mediated via altered thioredoxin activity. Lab Invest 2016-03-01 [PMID: 26692290] (IHC-P, PLA, WB, Human)	IHC-P, PLA, WB	Human
Bronner DN, Abuaita BH, Chen X et al. Endoplasmic Reticulum Stress Activates the Inflammasome via NLRP3- and Caspase-2-Driven Mitochondrial Damage. Immunity 2015-09-15 [PMID: 26341399] (WB, Mouse)	WB	Mouse
Wang X, Li R, Wang X, Fu Q, Ma. Umbelliferone ameliorates cerebral ischemia-reperfusion injury via upregulating the PPAR gamma expression and suppressing TXNIP/NLRP3 inflammasome. Neurosci Lett. 2015-07-23 [PMID: 26071904]
Li Y, Yang J, Chen MH et al. Ilexgenin A inhibits endoplasmic reticulum stress and ameliorates endothelial dysfunction via suppression of TXNIP/NLRP3 inflammasome activation in an AMPK dependent manner. Pharmacol Res. 2015-09-01 [PMID: 26054569]
Zhao Y, Li Q, Zhao W et al. Astragaloside IV and cycloastragenol are equally effective in inhibition of endoplasmic reticulum stress-associated TXNIP/NLRP3 inflammasome activation in the endothelium. J Ethnopharmacol. 2015-07-01 [PMID: 25922268]
Li Y, Li J, Li S et al. Curcumin attenuates glutamate neurotoxicity in the hippocampus by suppression of ER stress-associated TXNIP/NLRP3 inflammasome activation in a manner dependent on AMPK. Toxicol. Appl. Pharmacol. 2015-03-16 [PMID: 25791922] (WB, Mouse)	WB	Mouse
Song J, Li J, Hou F et al. Mangiferin inhibits endoplasmic reticulum stress-associated thioredoxin-interacting protein/NLRP3 inflammasome activation with regulation of AMPK in endothelial cells. Metab. Clin. Exp. 2014-11-28 [PMID: 25499441] (WB, Human)	WB	Human
Wu J, Xu X, Li Y et al. Quercetin, luteolin and epigallocatechin gallate alleviate TXNIP and NLRP3-mediated inflammation and apoptosis with regulation of AMPK in endothelial cells. Eur J Pharmacol. 2014-12-15 [PMID: 25446924]
Joshi S, Wang W, Peck AB, Khan SR. Activation of the NLRP3 Inflammasome in Association with Calcium Oxalate Crystal Induced Reactive Oxygen Species in Kidneys. J Urol. 2015-05-01 [PMID: 25437532]
Saxena G, Chen J, Shalev A. Intracellular shuttling and mitochondrial function of thioredoxin-interacting protein. J Biol Chem;285(6):3997-4005. 2010-02-05 [PMID: 19959470]
Chen J, Cha-Molstad H, Szabo A, Shalev A. Diabetes induces and calcium channel blockers prevent cardiac expression of proapoptotic thioredoxin-interacting protein. Am J Physiol Endocrinol Metab;296(5):E1133-9. 2009-05-01 [PMID: 19258488]
Show All 38 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Saifudeen Ismael	IHC-Fr	Mouse	02/28/2019	View Summary Application Immunohistochemistry-Frozen Sample Tested Mouse brain Species Mouse Lot A2 Comments Comments Antibody dilution: 1:100, incubated overnight in 5%BSA + 0.2% Triton X 100.
Enlarge	4 reviewed by: Saifudeen Ismael	WB	Mouse and mouse brain	08/22/2018	View Summary Application Western Blot Sample Tested Mouse brain cortex whole lysate,Mouse brain Species Mouse and mouse brain Lot A2
Enlarge	5 reviewed by: Chad Brocker	WB	Mouse	05/22/2018	View Summary Application Western Blot Sample Tested Hepa1 cells transfected with mouse TXNIP expression vector,Hepa1 whole cell lysates Species Mouse Lot 036 Comments Comments Hepa1 lysates probed with anti-TXNIP (JY2) @ 1:2K. Cells were transfected with either empty vector or mouse TXNIP expression vector (MYC/DDK tagged). Loaded 30ug protein per lane.

Array NBP1-54578

• TXNIP Antibodies
• TXNIP ELISA Kits
• TXNIP Lysate
• TXNIP Protein