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Trypsin 1/PRSS1 Antibody

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Product Details

Summary
Reactivity BvSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Trypsin 1/PRSS1 Antibody Summary

Description
This antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Trypsin [Bovine Pancreas]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Immunogen
Trypsin 1/PRSS1 1/PRSS1 [Bovine Pancreas] (Uniprot: P00760)
Localization
Secreted
Specificity
Trypsin
Isotype
Serum
Clonality
Polyclonal
Host
Rabbit
Gene
PRSS1
Purity
Delipidation and Defibrination
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:5000-1:25000
  • Western Blot 1:500-1:3000
Application Notes
This product has been assayed against 1.0 ug of Trypsin [Bovine Pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for This product . Findings were obtained using Costar Brand Vinyl Assay Plates #09997011 in NaHCO3 buffer at pH 8.0.
Publications
Read Publication using NB600-1277.

Reactivity Notes

Cross reactivity against Trypsin 1/PRSS1 from other tissues and species may occur but have not been specifically determined.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Delipidation and Defibrination

Alternate Names for Trypsin 1/PRSS1 Antibody

  • Beta-trypsin
  • Cationic trypsinogen
  • digestive zymogen
  • DKFZp779A0837
  • EC 3.4.21
  • EC 3.4.21.4
  • FLJ57296
  • MGC120175
  • MGC149362
  • nonfunctional trypsin 1
  • protease, serine, 1 (trypsin 1)
  • PRSS1
  • PTRYI
  • Serine protease 1
  • TRP1
  • TRY1beta-trypsin
  • TRY4
  • Trygn16
  • TRYP1
  • Trypsin 1
  • Trypsin I
  • trypsin-1
  • Trypsinogen 1
  • trypsinogen A

Background

The Trypsin gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of l

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Trypsin 1/PRSS1 Antibody (NB600-1277)(1)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRSS1
Uniprot