Trypsin 1/PRSS1 Antibody (A16-N) Summary
Description |
This antibody is immunoaffinity purified with immunogenic peptide as a ligand. |
Additional Information |
Recombinant Monoclonal Antibody. |
Immunogen |
Peptide derived from N-terminal sequence of human Trypsin 1/PRSS1. Antibody recognizes the epitope located between Lys92 - Ans106. |
Epitope |
Lys92 - Ans106 |
Specificity |
This antibody is specific for the N terminus of human pancreatic cationic trypsin.
Due to the high degree of homology between human trypsins, cross-detection with other trypsins than PRSS1 is possible |
Isotype |
IgG |
Clonality |
Monoclonal |
Host |
Rabbit |
Gene |
PRSS1 |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA 1:20000-1:50000
- Western Blot 1:4000
|
Application Notes |
For western blots, incubate the membrane with antibody diluted in blocking buffer 2 hours at room temperature. Western immunoblotting solutions: Wash buffer: 1x Tris Buffered Saline (TBS); 0.2% Tween-20. - Blocking buffer: 1xTBS; 0.2% Tween-20; 8% nonfat dry milk. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA |
Preservative |
0.05% Sodium Azide |
Purity |
Immunogen affinity purified |
Notes
This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
Alternate Names for Trypsin 1/PRSS1 Antibody (A16-N)
Background
This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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