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Trypsin 1/PRSS1 Antibody (A16-N)

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Western Blot: Trypsin Antibody (A16-N) [NBP1-30135] - Trypsin-human pancreas.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
A16-N
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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Trypsin 1/PRSS1 Antibody (A16-N) Summary

Description
This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Peptide derived from N-terminal sequence of human Trypsin 1/PRSS1. Antibody recognizes the epitope located between Lys92 - Ans106.
Epitope
Lys92 - Ans106
Specificity
This antibody is specific for the N terminus of human pancreatic cationic trypsin.

Due to the high degree of homology between human trypsins, cross-detection with other trypsins than PRSS1 is possible
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
PRSS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:20000-1:50000
  • Western Blot 1:4000
Application Notes
For western blots, incubate the membrane with antibody diluted in blocking buffer 2 hours at room temperature. Western immunoblotting solutions: Wash buffer: 1x Tris Buffered Saline (TBS); 0.2% Tween-20. - Blocking buffer: 1xTBS; 0.2% Tween-20; 8% nonfat dry milk.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for Trypsin 1/PRSS1 Antibody (A16-N)

  • Beta-trypsin
  • Cationic trypsinogen
  • digestive zymogen
  • DKFZp779A0837
  • EC 3.4.21
  • EC 3.4.21.4
  • FLJ57296
  • MGC120175
  • MGC149362
  • nonfunctional trypsin 1
  • protease, serine, 1 (trypsin 1)
  • PRSS1
  • PTRYI
  • Serine protease 1
  • TRP1
  • TRY1beta-trypsin
  • TRY4
  • Trygn16
  • TRYP1
  • Trypsin 1
  • Trypsin I
  • trypsin-1
  • Trypsinogen 1
  • trypsinogen A

Background

This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PRSS1
Entrez
Uniprot