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Troponin I Type 3 (cardiac) Antibody

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Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-20734] - Troponin I Type 3 (cardiac) antibody detects Troponin I Type 3(cardiac) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Green: Troponin I Type 3 (cardiac) stained by Troponin I Type 3 (cardiac) antibody diluted at 1:250. Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-20734] - Troponin I Type 3 (cardiac) antibody detects Troponin I Type 3(cardiac) protein at cytoplasm by immunohistochemical analysis. Sample: ...read more
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-20734] - Paraffin-embedded rat heart dilution: 1:500.
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-20734] - Paraffin-embedded mouse heart dilution: 1:500.
Western Blot: Troponin I Type 3 (cardiac) Antibody [NBP2-20734] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Troponin I Type 3 (cardiac) antibody [N1C3] ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, FeSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Troponin I Type 3 (cardiac) Antibody Summary

Immunogen
Full length human Troponin I Type 3 (cardiac) Recombinant protein.
Predicted Species
Porcine (93%), Bovine (92%), Feline (94%), Canine (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TNNI3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-20734.

Reactivity Notes

Cat (94%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for Troponin I Type 3 (cardiac) Antibody

  • Cardiac troponin I
  • CMD1FF
  • CMD2A
  • CMH7MGC116817
  • CTnI
  • familial hypertrophic cardiomyopathy 7
  • RCM1
  • TNNC1
  • TNNC1troponin I, cardiac
  • TNNI3,
  • troponin I type 3 (cardiac)
  • troponin I, cardiac muscle
  • Troponin I/TNNI3

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Troponin I Type 3
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NBP2-20734
Troponin I Type 3 (cardiac) Antibody
Species: Hu, Mu, Rt, Po, Bv, Ca, Fe
Applications: WB, ICC/IF, IHC
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Troponin I Type 3 (cardiac) Antibody (NBP2-20734)(1)

We have publications tested in 1 application: IHC.


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Product General Protocols

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Secondary Antibodies

 

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Blogs on Troponin I Type 3 (cardiac).

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Troponin I Type 3 (TnI, cardiac)
Troponin I is part of a heteromeric thin filament regulatory complex that governs skeletal and cardiac muscle contraction. Troponin I is composed of three subunits: troponin I (TnI), troponin T (TnT), and troponin C (TnC), and each of these components...  Read full blog post.
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Bioinformatics

Gene Symbol TNNI3
Uniprot