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Tripeptidyl-Peptidase I/TPP1 Antibody

Images

 
Western Blot: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-31758] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-31758] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-31758] - Mouse liver. TPP1 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, Mu, Bv, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Tripeptidyl-Peptidase I/TPP1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Tripeptidyl-Peptidase I/TPP1. The exact sequence is proprietary.
Localization
Lysosome, Melanosome
Predicted Species
Bovine (91%), Chimpanzee (100%), Canine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TPP1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Tripeptidyl-Peptidase I/TPP1 Antibody

  • Cell growth-inhibiting gene 1 protein
  • ceroid-lipofuscinosis, neuronal 2, late infantile (Jansky-Bielschowsky disease)
  • CLN2
  • CLN2EC 3.4.14.9
  • growth-inhibiting protein 1
  • LINCL
  • LPIC
  • lysosomal pepstatin insensitive protease
  • Lysosomal pepstatin-insensitive protease
  • MGC21297
  • TPP1
  • TPP-1
  • TPP-I
  • Tripeptidyl aminopeptidase
  • tripeptidyl peptidase I
  • tripeptidyl-peptidase 1
  • TripeptidylPeptidase I
  • Tripeptidyl-Peptidase I

Background

This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TPP1