Transportin 1 Antibody (D45) - Azide and BSA Free

Images

 
Western Blot: Transportin 1 Antibody (D45) - Azide and BSA Free [NBP2-80991] - Whole cell protein from A431, Hek293 and MCF7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunohistochemistry-Paraffin: Transportin 1 Antibody (D45) - Azide and BSA Free [NBP2-80991] - IHC analysis of a formalin fixed paraffin embedded tissue section of human pancreas using Transportin 1 antibody (clone ...read more
Western Blot: Transportin 1 Antibody (D45) - Azide and BSA Free [NBP2-80991] - Human lung cancer cell line usig lysates from HCC44 cell line. (N: Nuclear fraction, C: Cytoplasmic fraction). Image from verified customer ...read more
Immunohistochemistry-Paraffin: Transportin 1 Antibody (D45) - Azide and BSA Free [NBP2-80991] - IHC analysis of a formalin fixed paraffin embedded tissue section of human pancreas using Transportin 1 antibody (clone ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, Ca, Pm, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, MA, CyTOF-ready
Clone
D45
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

Transportin 1 Antibody (D45) - Azide and BSA Free Summary

Immunogen
Human recombinant His-tagged Transportin 1. [UniProt# Q92973]
Localization
Cytoplasm. Nucleus.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNPO1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 9298975)
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000. Use reported in scientific literature (PMID 24647604)
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1:1000
Application Notes
WB: Detects a band of approximately 93 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 15738418).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Transportin 1 Antibody (D45) - Azide and BSA Free

  • importin beta 2
  • Importin beta-2
  • IPO2
  • karyopherin (importin) beta 2
  • MIP1Karyopherin beta-2
  • MIPM9 region interaction protein
  • transportin 1
  • transportin-1
  • TRNKPNB2importin 2

Background

Heterogeneous nuclear ribonucleoprotein hnRNPA1 is an abundant nuclear protein that plays an important role in pre-mRNA processing and mRNA export from the nucleus. A1 shuttles rapidly between the nucleus and the cytoplasm, and a 38-amino acid domain, M9, serves as the bi-directional transport signal of A1. Recently, a 90-kD protein, transportin, was identified as the mediator of A1 nuclear import. Transportin mediates the nuclear import of additional hnRNP proteins, including hnRNPF. The nuclear localization of A1 is dependent on ongoing RNA polymerase II transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TNPO1