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Transglutaminase 3/TGM3 Antibody [Unconjugated]

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Western blot shows lysates of B16-F1 mouse melanoma cell line, Bowes human melanoma cell line, and human skin tissue. PVDF Membrane was probed with 1 µg/mL of Human Transglutaminase 3/TGM3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4604) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, Recombinant Human Transglutaminase 3/TGM3 (Catalog # 4604-TG) (10 ng/lane) uncleaved and cleaved by recombinant human Cathepsin were included. Specific bands were detected for uncleaved Transglutaminase 3/TGM3 at approximately 77 kDa (as indicated) and Transglutaminase 3/TGM3 cleavage products at approximately 50 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of B16-F1 mouse melanoma cell line, Bowes human melanoma cell line, and human skin tissue. PVDF Membrane was probed with 1 µg/mL of Human Transglutaminase 3/TGM3 Antigen Affinity-purified ...read more
Transglutaminase 3/TGM3 was detected in immersion fixed paraffin-embedded sections of human esophagus using Human Transglutaminase 3/TGM3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4604) at 10 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4604-SP
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Transglutaminase 3/TGM3 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 3/TGM3
Ala2-Glu693
Accession # NP_003236
Specificity
Detects human Transglutaminase 3/TGM3 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
TGM3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Transglutaminase 3/TGM3 Antibody [Unconjugated]

  • EC 2.3.2.13
  • MGC126250
  • protein-glutamine gamma-glutamyltransferase E
  • TG(E)
  • TG3
  • TGase E
  • TGase-3
  • TgaseE
  • TGEMGC126249
  • TGM3
  • transglutaminase 3 (E polypeptide, protein-glutamine-gamma-glutamyltransferase)
  • Transglutaminase 3
  • Transglutaminase E
  • transglutaminase-3

Background

Transglutaminase 3 (TG3), also known as epidermal Transglutaminase (Tgase E), belongs to the family of Transglutaminase enzymes that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1-3). TG3 is involved in the formation of the cornified envelope in skin keratinocytes (4). It functions to cross-link structural proteins during epidermal terminal differentiation. TG3 has been implicated as the dominant autoantigen in dermatitis herpetiformis (5). TG3 activation requires proteolysis of the 77 kDa zymogen into two fragments of approximately 50 and 27 kDa to form the active enzyme (1).
  1. Kim, I.G. et al. (1993) J. Biol. Chem. 268:12682.
  2. Griffin, M. et al. (2002) Biochem. J. 368:377.
  3. Lorand, L. and R.M. Graham (2003) Nat. Rev. Mol. Cell Biol. 4:140.
  4. Eckert, R.L. et al. (2005) J. Invest. Dermatol. 124:481.
  5. Sardy, M. et al. (2002) J. Exp. Med. 195:747.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TGM3
Uniprot