![Western Blot: transgelin 2 Antibody [NBP2-20697] - Wild-type (WT) and Transgelin 2 knockout (KO) 293T cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/transgelin-2-Antibody-Western-Blot-NBP2-20697-img0009.jpg "Western Blot: transgelin 2 Antibody [NBP2-20697] - Wild-type (WT) and Transgelin 2 knockout (KO) 293T cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human transgelin 2. The exact sequence is proprietary. |
| Predicted Species | Rat (96%), Rhesus Macaque (99%), Bovine (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TAGLN2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: transgelin 2 Antibody [NBP2-20697] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/transgelin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20697-img0008.jpg "Immunocytochemistry/Immunofluorescence: transgelin 2 Antibody [NBP2-20697] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: transgelin 2 Antibody [NBP2-20697] - Paraffin-embedded mouse prostate. Transgelin 2 antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/transgelin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-20697-img0006.jpg "Immunohistochemistry-Paraffin: transgelin 2 Antibody [NBP2-20697] - Paraffin-embedded mouse prostate. Transgelin 2 antibody [N1C3] diluted at 1:500.")
![Western Blot: transgelin 2 Antibody [NBP2-20697] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/transgelin-2-Antibody-Western-Blot-NBP2-20697-img0002.jpg "Western Blot: transgelin 2 Antibody [NBP2-20697] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: transgelin 2 Antibody [NBP2-20697] - Analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/transgelin-2-Antibody-Western-Blot-NBP2-20697-img0004.jpg "Western Blot: transgelin 2 Antibody [NBP2-20697] - Analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: transgelin 2 Antibody [NBP2-20697] - A549 cells were fixed in ice-cold MeOH for 5 min. Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/transgelin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20697-img0007.jpg "Immunocytochemistry/Immunofluorescence: transgelin 2 Antibody [NBP2-20697] - A549 cells were fixed in ice-cold MeOH for 5 min. Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: transgelin 2 Antibody [NBP2-20697] - : Paraffin-embedded mouse pancreas. Transgelin 2 antibody [N1C3] diluted 1:500.](http://images.novusbio.com/fullsize/transgelin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-20697-img0005.jpg "Immunohistochemistry-Paraffin: transgelin 2 Antibody [NBP2-20697] - : Paraffin-embedded mouse pancreas. Transgelin 2 antibody [N1C3] diluted 1:500.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")