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TPPP/p25 Antibody

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Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833] - Staining of Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) Analysis of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833] - Negative Control showing staining of Human Cerebellum, with no primary antibody.
Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833] - (2ug/ml) staining of Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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TPPP/p25 Antibody Summary

Immunogen
Peptide with sequence C-DPSKDRAAKRLSLES corresponding to internal region according to NP_008961.1.
Epitope
DPSKDRAAKRLSLES - Internal region
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TPPP
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.0001-0.01 ug/ml
Application Notes
WB: Approx. 26 kDa band observed in human brain is reported in scientific literature (PMID: 18028908). An additional band of unknown identity was also consistently observed at 17 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human cerebral cortex shows cytoskeletal-like staining throughout the fibre network.
Publications
Read Publications using NBP1-49833.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TPPP/p25 Antibody

  • p24
  • P24,25 kDa brain-specific protein
  • p25
  • p25alpha
  • p25-alpha
  • TPPP/p25brain specific protein p25 alpha
  • TPPP1glycogen synthase kinase 3 (GSK3) inhibitor p24
  • tubulin polymerization promoting protein
  • tubulin polymerization-promoting protein

Background

TPPP may play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity ofthe microtubule network. May play a role in mitotic spindle assembly and nuclear env

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TPPP/p25 Antibody
NBP1-49833
TPPP/p25 Antibody
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Applications: WB, ELISA, IHC

Publications for TPPP/p25 Antibody (NBP1-49833)(2)


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Bioinformatics

Gene Symbol TPPP
Entrez
Uniprot