TLR9 Antibody (eB72-1665) - BSA Free

eB72-1665 is generated against a portion of human toll-like receptor 9 (aa 273-288), a molecule reported to be expressed predominantly intracellularly. TLR9 is a ~115-120 kDa molecule, which mediates response to unmethylated CpG dinucleotides in bacterial DNA. CpG DNA induces a strong T-helper-1-like inflammatory response and the proliferation of TLR9-positive human B cells. When stimulated with CpG DNA, TLR9-deficient (TLR9-/-) mice lacked splenocyte proliferation, inflammatory cytokine production from macrophages, and dendritic cell maturation, as compared with normal mice. To date, at least twelve members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Members of the TLR family are involved in recognition and response to different microbial components including lipoproteins, peptidoglycans, and nucleic acids and play important roles in innate immunity and inflammation. TLR9 is not detected by flow cytometry using this antibody on lysed whole human blood and/or isolated human PBMC stained for cell surface or intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Human pDCs matured in the presence of IL-3 have been reported to stain with eB72-1665 by immunofluorescence microscopy. Human Epithelial Cell lines were also reported to stain with this mAb. Further studies are needed to determine the relationship between mRNA expression and protein detection by flow cytometry.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 application: FLOW.

Showing Publications 1 - 6 of 6.

Publications using NBP1-43140	Applications	Species
Dias AT, de Castro SBR, de Souza Alves CC et al. Genistein modulates the expression of Toll-like receptors in experimental autoimmune encephalomyelitis. Inflamm Resow 2018-04-23 [PMID: 29687146] (FLOW) Details: This citation used the PE version of this antibody.	FLOW
Peduzzi E, Groeper C, Schutte D et al. Local activation of the innate immune system in Buruli ulcer lesions. J Invest Dermatol. 2007-03-01 [PMID: 17068481]
Jozsef L, Khreiss T, El Kebir D et al. Activation of TLR-9 induces IL-8 secretion through peroxynitrite signaling in human neutrophils. J Immunol. 2006-01-01 [PMID: 16394009]
Schroeder JT, Bieneman AP, Xiao H et al. TLR9- and FcepsilonRI-mediated responses oppose one another in plasmacytoid dendritic cells by down-regulating receptor expression. J Immunol. 2005-11-01 [PMID: 16237063]
Dasari P, Nicholson IC et al. Expression of toll-like receptors on B lymphocytes Cell Immunol 2005-01-01 [PMID: 16188245] Details: This citation used the PE version of this antibody.
Leifer CA, Kennedy MN, Mazzoni A et al. TLR9 is localized in the endoplasmic reticulum prior to stimulation. J Immunol. 2004-07-01 [PMID: 15240708]

Array NBP1-43140

• TLR9 Antibodies
• TLR9 ELISA Kits
• TLR9 Proteins
• TLR9 Proteins and Enzyme