![Western Blot: TLR9 Antibody (2A4C6.2E5) [NBP2-31150] - Mouse Liver (lane 1), Mouse Kidney (lane 2) and Mouse Spleen (lane 3) were separated by SDS-PAGE and the protein transferred to PVDF. The membrane was then probed with anti-TLR9 at 2ug/mL and detected with an anti-rat HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/TLR9-Antibody-2E5-Western-Blot-NBP2-31150-img0007.jpg "Western Blot: TLR9 Antibody (2A4C6.2E5) [NBP2-31150] - Mouse Liver (lane 1), Mouse Kidney (lane 2) and Mouse Spleen (lane 3) were separated by SDS-PAGE and the protein transferred to PVDF. The membrane was then probed with anti-TLR9 at 2ug/mL and detected with an anti-rat HRP secondary antibody using chemiluminescence.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, IHC, ICC/IF (-) |
| Clone | 2A4C6.2E5 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Partial recombinant protein made to an internal portion of mouse TLR9 (between amino acids 500-800) [UniProt Q9EQU3] |
| Localization | Endoplasmic reticulum membrane, Endosome, Lysosome, Cytoplasmic vesicle/phagosome. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | TLR9 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Agonist |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for TLR9 Antibody (NBP2-31150)Find related products by research area.
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Toll-like receptors in the intestinal epithelial cells By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ... Read full blog post. |
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Topics in CD11b: The innate immune response Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens. Integrin... Read full blog post. |
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MHC Class I and the Herpes Simplex Virus MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens. These molecules are highly polymorphic, meaning that they exist in multiple varian... Read full blog post. |
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The role of TLR4 in breast cancer Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation. In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy... Read full blog post. |
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IRAK4: The "master IRAK" critical for initiating immune responses IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec... Read full blog post. |
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TLR9: For Whom the Cell Tolls The Toll-like receptor 9 (TLR9) protein, also known as CD289, belongs to the family of Toll-like receptor (TLR) proteins which play a large role in pathogen recognition and the activation of innate immunity. Scientists using TLR9 antibodies have found... Read full blog post. |
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TLR9: Tollgate to Immunity Toll-like receptors (TLRs) play an essential role in the activation of innate immunity, and TLRs are expressed in a large number of immune cells as well as in epithelial cells. TLR9 recognizes synthetic oligodeoxynucleotides (ODN) containing unmethyla... Read full blog post. |
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TLR9, Infectious Disease and Cancer Toll-like receptor 9 (TLR9) is a protein encoded by TLR9 gene in humans. It is also known as cluster of differentiation 289 (CD289) and is a member of TLR family. Proteins from TLR family are transmembrane proteins that expressed in both antigen-resen... Read full blog post. |
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TLR9 Antibodies in Immunity Research Toll-like receptor 9 (TLR9) is a member of the toll-like receptor family that plays a key role in pathogen recognition and activation of innate immunity. Scientists using TLR9 antibodies have found the protein is highly conserved from Drosophila to hu... Read full blog post. |
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![Immunohistochemistry-Paraffin: TLR9 Antibody (2A4C6.2E5) [NBP2-31150] - Analysis of TLR9 protein in normal mouse epididymis section using TLR9 antibody (clone 2E5) at a concentration of 5 ug/mL. This antibody developed strong cytoplasmic staining in the pseudostratified columnar epithelial cells as well as the blood vessels of epididymis. The basal cells, the smooth muscle cells and the mature sperm cells were largely found negative for TLR9 staining.](http://images.novusbio.com/fullsize/TLR9-Antibody-2E5-Immunohistochemistry-Paraffin-NBP2-31150-img0006.jpg "Immunohistochemistry-Paraffin: TLR9 Antibody (2A4C6.2E5) [NBP2-31150] - Analysis of TLR9 protein in normal mouse epididymis section using TLR9 antibody (clone 2E5) at a concentration of 5 ug/mL. This antibody developed strong cytoplasmic staining in the pseudostratified columnar epithelial cells as well as the blood vessels of epididymis. The basal cells, the smooth muscle cells and the mature sperm cells were largely found negative for TLR9 staining.")
![Western Blot: TLR9 Antibody (2A4C6.2E5) [NBP2-31150] - TLR9 Antibody (2E5) [NBP2-31150] - TLR9 Antibody (2E5) [NBP2-31150] - WB analysis of partial recombinant TLR9 protein (expected molecular weight ~21kDa) with TLR9 antibody (clone 2A4C6.2E5) at a concentration of 2 ug/mL.](http://images.novusbio.com/fullsize/TLR9-Antibody-2E5-Western-Blot-NBP2-31150-img0004.jpg "Western Blot: TLR9 Antibody (2A4C6.2E5) [NBP2-31150] - TLR9 Antibody (2E5) [NBP2-31150] - TLR9 Antibody (2E5) [NBP2-31150] - WB analysis of partial recombinant TLR9 protein (expected molecular weight ~21kDa) with TLR9 antibody (clone 2A4C6.2E5) at a concentration of 2 ug/mL.")
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![CpG oligodeoxynucleotides with negative control, TLR9 ligand [NBP2-26232] - Validation of CpG using the TLR9/HEK 293 cell line. The assay was performed using the NF-kB SEAPorter Assay Kit. The Vector/HEK 293 and TLR9/HEK 293 cell lines were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with various amounts of CpG for 24 h followed by SEAP assay.](https://images.novusbio.com/images/CpG-oligodeoxynucleotides-with-negative-control--TLR9-ligand-NBP2-26232-img0002.jpg "CpG oligodeoxynucleotides with negative control, TLR9 ligand [NBP2-26232] - Validation of CpG using the TLR9/HEK 293 cell line. The assay was performed using the NF-kB SEAPorter Assay Kit. The Vector/HEK 293 and TLR9/HEK 293 cell lines were transfected with NF-kB/SEAP reporter plasmid for 16 h. Cells were stimulated with various amounts of CpG for 24 h followed by SEAP assay.")